Robert H Chow

6 Questions 23 Answers 0 Followers

Questions related from Robert H Chow

Do people have a favourite cell line that they use to teach patch clamp or to test a newly built patch clamp rig?

I’m looking for a good cell line to use to teach undergraduate students patch clamp. The main trait is that they should be easy to get gigaseals on. Ideally that should also have reasonable large...

28 November 2024 8,212 2 View

Does anyone know where one can buy 8161 glass capillaries for making patch pipettes?

Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore

05 June 2024 5,777 2 View

Using patch clamp with sharp electrodes?

Has anyone tried using their patch clamp amplifier in current clamp mode to make intracellular voltage recording with sharp electrodes? We’re having major difficulty getting gigaseals so...

25 October 2023 7,029 3 View

Can anyone suggest a fluorescent dye that can be loaded into neurons during patch clamp, and that will stay in the neurons after fixing the tissue?

We want to perform image analysis to characterize neuron branching, post patch clamp experiment, in fixed neurons.

28 November 2017 1,185 8 View

Can anyone suggest a free or inexpensive deconvolution software package that gives good results?

It's really the complete system that we need, including focusing device, needed for 3D reconstruction of neurons and processes in brain slices

31 March 2014 9,098 5 View

Is there a "gold standard" preparation for electron microscopy of clathrin coated pits in endocytosis?

We are imaging what appears to be endocytosis in photoreceptors, and we want a "positve control" for what we are seeing.

05 March 2013 5,417 0 View