How to produce neurospheres from parental glioma cell lines?

Hello, We are currently trying to produce neuerosphere cultures from parental glioma cells lines. Parental cells were disassociated and cultured in neurobasalA serum free media containing...

02 March 2019 2,542 2 View

What are the most important publications in biology/molecular biology in the last 100 years?

Hi, If you could name the most important publications relating to any of the biological sciences over the last 100 years what would those be? Beginning with Francis & Crick's paper on...

06 July 2018 5,683 4 View

What is causing white smears on my western blot?

Hey Guys, I performed a western at 90 volts for 1 hr 30min, transfer using iblot dry transfer system to a PVDF membrane, 1 hour block in 5% milk and incubated with primary overnight in BSA and...

31 December 2017 8,349 3 View

Why do my bands not appear correctly on my western membrane?

Hey guys! I hope everyone is well, I ran a protein western probing for LC3 antibody. When I image my gel the bands from my treated samples do not appear as straight lines, rather 'n' shaped bands...

10 November 2017 6,193 3 View

How can I determine the rate of protein synthesis following ribosome biogenesis inhibition?

Hi, I am treating my cells with a RNA Pol inhibitor which inhibits transcription of rDNA. Ribosome production will be inhibited and I would like to show how this affects the rate of protein...

31 December 2016 5,986 3 View

How to prepare drug concentrations for MTS assay?

Hello, I would like to perform an MTS assay using the following concentrations: 100uM, 50uM, 20uM, 10uM, 5uM, 2uM, 1uM and 0.5uM. I will be adding 100ul of each of my drug doses to a well on a 96...

09 October 2015 9,150 1 View

How can I choose fluorescent antibody for cell imaging?

Hello, I am attempting to image the three compartments of the nucleolus. I have chosen RNA Pol1 as a marker for the fibrillar centre, fibrillarin as a marker for the dense fibrillar centre and B23...

09 October 2015 2,512 6 View

Which melanoma cell line has good nucleolar morphology?

09 October 2015 2,419 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View