Mohammed Ismael

9 Questions 11 Answers 0 Followers

Questions related from Mohammed Ismael

How to produce neurospheres from parental glioma cell lines?

Hello, We are currently trying to produce neuerosphere cultures from parental glioma cells lines. Parental cells were disassociated and cultured in neurobasalA serum free media containing...

03 March 2019 2,684 2 View

What are the most important publications in biology/molecular biology in the last 100 years?

Hi, If you could name the most important publications relating to any of the biological sciences over the last 100 years what would those be? Beginning with Francis & Crick's paper on...

07 July 2018 5,899 4 View

What is causing white smears on my western blot?

Hey Guys, I performed a western at 90 volts for 1 hr 30min, transfer using iblot dry transfer system to a PVDF membrane, 1 hour block in 5% milk and incubated with primary overnight in BSA and...

01 January 2018 8,480 3 View

Why do my bands not appear correctly on my western membrane?

Hey guys! I hope everyone is well, I ran a protein western probing for LC3 antibody. When I image my gel the bands from my treated samples do not appear as straight lines, rather 'n' shaped bands...

11 November 2017 6,329 3 View

How can I determine the rate of protein synthesis following ribosome biogenesis inhibition?

Hi, I am treating my cells with a RNA Pol inhibitor which inhibits transcription of rDNA. Ribosome production will be inhibited and I would like to show how this affects the rate of protein...

01 January 2017 6,121 3 View

Is there a Glioblastoma cell line with p53 positive status?

Hello, I am currently working with LN18 which has a p53 mutated status. I would also like to experiment on another glioblastoma cell line which has a p53 non mutated status, can anyone recommend a...

10 October 2015 9,271 5 View

Which melanoma cell line has good nucleolar morphology?

Hello, Anyone know of any good melanoma cell lines which display clear, distinguishable (and preferably large) nucleoli and also with known p53 status please? Thank you

10 October 2015 2,551 3 View

How can I choose fluorescent antibody for cell imaging?

Hello, I am attempting to image the three compartments of the nucleolus. I have chosen RNA Pol1 as a marker for the fibrillar centre, fibrillarin as a marker for the dense fibrillar centre and B23...

10 October 2015 2,644 6 View

How to prepare drug concentrations for MTS assay?

Hello, I would like to perform an MTS assay using the following concentrations: 100uM, 50uM, 20uM, 10uM, 5uM, 2uM, 1uM and 0.5uM. I will be adding 100ul of each of my drug doses to a well on a 96...

10 October 2015 9,338 1 View