Does failure have any value?

31 December 2018 8,799 10 View

Are brain lacunar infarctions thrombotic?

The nature of lacunar and non-lacunar brain infarcts continues to elude investigators busy in piling up data. Does all single infarction have lower risk of stroke recurre... This is not merely...

31 December 2018 7,314 6 View

Medicine is not an appendage of technology: true or false?

Such issues arise when we start thinking through technology, and, expect technology to do the hard work for us. Will technology oblige and make clinical medicine its appendage. Or will technology...

11 December 2018 5,355 19 View

What would migraine and other primary headache scientists like to forget?? And why????

1. Lateralization of headache. 2. Blood-brain barrier. 3. Plasma glutamate cannot freely cross the BBB. 4. Plasma magnesium cannot freely cross the BBB. 5. Cortical spreading depression till...

11 December 2018 1,252 4 View

Does peripherally released glutamate freely cross the intact blood-brain barrier in humans?

Glutamate has attracted lot of attention in neuroscience and migraine research. It is time to come to grips with this issue.

10 November 2018 7,196 5 View

What is ghost authorship?

Would you participate as a ghost scientific author? What are the advantages and disadvantages from ghost authorship? How does it impact science and human welfare? What is the ethical aspect of...

09 October 2018 7,958 0 View

What wins the Nobel /Lasker prize -- quantity or quality of publication?

Obviously, it is NOT both.

09 October 2018 1,269 10 View

Why does the anchor place her/his name last or first in the list of citations?

Is it related to the certitude or the uncertainty of the article? Or is there some sense to this nonsense?

08 September 2018 1,275 5 View

Is scientific discovery a multi-part random act, a primal action of inspiration, or data crunching?

07 August 2018 3,376 1 View

Is scientific research, a re-search?

Are the waves already out there? Ready for capture?

04 May 2018 9,685 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View