Which is the best the detector in HPLC for test cananbinoids?

In the HPLC, we can use differents detector methods to quantify the cannabinoid content from the flowers of Cannabis sativa. Most populars detectors are: 1. UV 2. Diodo Array Detector 3. Mass...

19 September 2019 459 3 View

BD CBA cytokine kit assay use with BDFACSDiva and Flowjo?

Does anyone know if the CBA cytokine assay offered by BDbiosciences is compatible to run with BDFACSDiva software on a flow cytometer (vs the BDFACSSuite software that is recommended in the user...

15 January 2019 5,663 5 View

What is the best way to convert Multiband sequence DICOMs into Nifti?

Normally for functional images in the past I've used Xiangrui Li's Matlab script or mricron's dcm2nii but this is the first time I am working with Multiband data and have been running into...

12 April 2018 5,553 3 View

When performing multicolor flow cytometry can one use several different color panels in one experiment?

So let's say I have 3 samples and I am interested in testing different fluorophore-conjugated antibodies against certain antigens by using 12 different colors. Howeveer, aach sample only has 10...

05 April 2018 4,083 2 View

Why do I get fluctuating peak areas?

I carried out an experiment aimed at confirming the presence of aspirin and to quantify the abundance of asprin using standard addition. And using HPLC instrumentation. From the result, the...

05 April 2018 5,916 6 View

ELISA assays: Negative results for known positives?

I am testing insoluble homogenates diluted to 0.25M guanidine HCL. Standards for every kit tested work beautifully (standards also in guanidine HCL dilution. The samples are from human gene KI...

22 January 2018 8,822 2 View

When staining targets from 2 different species for flow cytometry, can you use 2 different primary Abs against different species in the same sample?

(if you staining for a mouse target and human target within one mouse (ex, chimera), can this be stained in the same tissue sample? Or should samples be stained separately?)

09 August 2017 7,149 2 View

Can anyone explain why I get a lower effect estimate using two-stage logistic analyses compared to straightforward logistic regression?

I am using a two-stage approach (whereby admissions to hospital are nested within patients) to account for multiple admissions of individual patients during a study period. Intuitively it makes...

21 September 2016 8,379 4 View

Are there available ID keys to marine epiphytes associated with seagrasses?

16 August 2016 395 1 View

Can anyone recommend a good DNA ladder that can be used for products from 100bp to 20kb?

I am looking for an easy to read ladder especially on the upper end. I have a ladder that currently goes up to 12kb but after 5kb the bands are hard to decipher.

13 January 2016 3,167 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View