Is there a difference in cord blood between deliveries?

More Akshaya Keerthi's questions See All

What is meant by Hits (DB) in High-Resolution Liquid Chromatography-Mass spectrometry (HR-LCMS) analysis compound table report?

In the qualitative compound report obtained from HR-LCMS analysis of crude plant extracts, what is meant by Hits (DB)? Should we consider all the predicted compounds from the list for further studies?

30 June 2024 2,290 4 View

Can 0.1% ammonium hydroxide damage HILIC column?

In my LC-MS experiment, I am utilizing an Agilent Polaris NH2 column to separate a mixture of glucose, fructose, and sucrose. I am using water and acetonitrile as A and B mobile phases...

26 March 2024 8,895 4 View

Can ProSA web server be used to analyze the protein structures downloaded from Protein Data Bank?

Or is it used only to analyze the modelled protein structures.

02 March 2024 6,662 2 View

How to visualize interactions of protein-protein docked model obtained from ClusPro web server?

Not able to view the downloaded model from ClusPro using PDBSum. Since both the docked proteins contain single A chain, PDBSum shows it as single image. Please give suggestions. Also tried to save...

18 January 2024 5,286 4 View

Why polymer like peaks with C30 column always?

I am using a C30 column for carotenoid analysis. I bought the column recently and using it for the first time. When I use the column in LC-MS scan mode, I clearly see the UV-Vis peaks for my...

08 June 2023 2,357 2 View

What is the possible peak at m/z = 535.8 for lycopene?

I am analyzing Lycopene using LC-MS. I use a C18 column and my mobile phase is Methanol: MTBE = 95:5. I use an Agilent Ultivo QQQ mass spectrometer with APCI negative ion mode. I see the precursor...

07 May 2023 525 0 View

Is the expression of active SARS COV2 of Membrane protein in bacterial system feasible?

I am trying to express the soluble fraction Membrane Protein SARS Cov2 in E. Coli. I am getting only monomers while it's the dimer form that is active. Literature that I have come across have used...

20 March 2023 4,459 1 View

What does "defined levels are outside of dataset range" mean in 2D NMR?

I am running an HMBC experiment on a Bruker 300 mHz instrument. I set up the experiment following the steps given in TopSpin guide book. Everything happens fine but when I try to open the spectrum...

09 March 2023 5,289 4 View

What is a suitable solid phase extraction method for fatty acid methyl esters?

I need to concentrate some fatty acid methyl esters in a cell culture medium to separate and isolate them using Prep-HPLC. What type of solid phase extraction would be suitable to concentrate...

02 March 2023 9,526 0 View

How can dsDNA be visualized with fluorescent dyes in a fluorescent microscope without performing PCR?

Hi, I'm looking for a clean and direct visualization method to image dsDNA (assume purified extract available) with a fluorescence microscope. Currently, the most common ways to "visualize"...

16 January 2023 4,525 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What researches are there for satisfaction level of the hospital attachment for student nurses ?

I am doing a study on nursing students satisfaction on the hospital attachment whether they are satisfied with the clinical attachment or not. I need more research studies on this

03 August 2024 6,985 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Recent topic in digital banking?

i need a recent digital banking topic for doing my Ph.d research

29 July 2024 5,210 2 View

Amira Othman

Dear Akshaya

https://www.researchgate.net/profile/Ahmad_Darwish

Please follw my collegue Ahmad Darwish , he is really expert in this area , his profile contains alot of his papers .

Good luck.

Gregory Barshtein

Dear Akshaya,

I hope that following publications will be helpful.

http://www.ijpbs.net/vol-3/issue-4/Bio/7.pdf

http://www.ncbi.nlm.nih.gov/pubmed/12106881