Why does silver nitrate turn brown upon addition of leaf extract but no increase in absorption at 400 - 500 nm?

I am currently biosynthesizing silver nanoparticles using ethanolic leaf extract. Upon addition of the leaf extract to 1mM silver nitrate, the solution turns brown in color after exposure to...

08 November 2020 1,009 6 View

How to present the results of a binary logistic regression for an ecological paper?

I conducted a binary logistic regression using the glm function in R. My binary predictor is the presence or absence of my study species. I have two significant variables but am just not sure what...

19 October 2020 5,398 3 View

Why do we shift fermi to zero in electronic band structures?

In most electronic structures the fermi level is always shifted to zero , why ?

26 April 2020 163 1 View

What is the relationship between Imagination and emotion ?

It seems to me that this relationship is foregrounded in for example Japanese arts, but that the two are often uncoupled , for example in arts in the UK? I'm not restricting my field to the arts,...

13 January 2020 773 3 View

What is the relationship between creativity and democracy?

We have just started ReCreaDe a 3 year project investigating the ways that informal and nonformal learning can widen the scope for understanding the relationship between democracy, diversity and...

02 May 2019 3,162 7 View

How to cryopreserve tobacco BY-2 cells?

There is a protocol by Menges and Murray (2004) Plant J 37, 635-644, but the main description is for Arabidopsis cells, and I cannot make out the exact protocol used for the BY-2 cells. Does...

17 January 2019 6,651 3 View

Is anyone working with Fabry cell lines?

I am currently looking for laboratories or cell line repositories that are working or have worked with Fabry disease who are willing to provide me some transformed Fabry cell lines preferably...

03 August 2017 1,805 2 View

Can i find a short (<1000bp), strong and ubiquitous promoter for drosophila in vivo ?

Many of theses promoter i can find are very long (>2500bp; actin5c or tubulin) and i need some space on my plasmid. So, is it possible to find a smaller promoter ?

16 February 2017 4,447 1 View

Can anyone help me with a data set containing windows API calls for use in detecting malware?

25 October 2016 1,910 2 View

Does anyone have a good protocol for using H2DCFDA to measure ROS in adherent cells as macrophages?

I'm culturing adherent cells (5 x 10^5 peritoneal macrophages and bone marrow-derived macrophages), stimulating them with some drugs and a pro-inflammatory stimulus (LPS) for 24hours. I normally...

27 January 2016 733 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View