Why does silver nitrate turn brown upon addition of leaf extract but no increase in absorption at 400 - 500 nm?

I am currently biosynthesizing silver nanoparticles using ethanolic leaf extract. Upon addition of the leaf extract to 1mM silver nitrate, the solution turns brown in color after exposure to...

08 November 2020 1,009 6 View

How to present the results of a binary logistic regression for an ecological paper?

I conducted a binary logistic regression using the glm function in R. My binary predictor is the presence or absence of my study species. I have two significant variables but am just not sure what...

19 October 2020 5,398 3 View

Why do we shift fermi to zero in electronic band structures?

In most electronic structures the fermi level is always shifted to zero , why ?

26 April 2020 163 1 View

What is the relationship between Imagination and emotion ?

It seems to me that this relationship is foregrounded in for example Japanese arts, but that the two are often uncoupled , for example in arts in the UK? I'm not restricting my field to the arts,...

13 January 2020 773 3 View

What is the relationship between creativity and democracy?

We have just started ReCreaDe a 3 year project investigating the ways that informal and nonformal learning can widen the scope for understanding the relationship between democracy, diversity and...

02 May 2019 3,162 7 View

How to cryopreserve tobacco BY-2 cells?

There is a protocol by Menges and Murray (2004) Plant J 37, 635-644, but the main description is for Arabidopsis cells, and I cannot make out the exact protocol used for the BY-2 cells. Does...

17 January 2019 6,651 3 View

Is anyone working with Fabry cell lines?

I am currently looking for laboratories or cell line repositories that are working or have worked with Fabry disease who are willing to provide me some transformed Fabry cell lines preferably...

03 August 2017 1,805 2 View

Can i find a short (<1000bp), strong and ubiquitous promoter for drosophila in vivo ?

Many of theses promoter i can find are very long (>2500bp; actin5c or tubulin) and i need some space on my plasmid. So, is it possible to find a smaller promoter ?

16 February 2017 4,447 1 View

Can anyone help me with a data set containing windows API calls for use in detecting malware?

25 October 2016 1,910 2 View

Does anyone have a good protocol for using H2DCFDA to measure ROS in adherent cells as macrophages?

I'm culturing adherent cells (5 x 10^5 peritoneal macrophages and bone marrow-derived macrophages), stimulating them with some drugs and a pro-inflammatory stimulus (LPS) for 24hours. I normally...

27 January 2016 733 4 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

Is nr blast important?

Hello, I have designed a specific primer for my Gene, when I blast this primer, it just finds the mRNA of the gene, but in nr blast, I have found some CDSs that are not relevant to my Gene. Do...

05 February 2021 5,842 3 View

Abaqus - plate is going up under gravity+blast loads?

Hi, I have modeled concrete plate with reinforcement supported on 2 concrete block. between concrete block and the plate I have added regular contact. I have created 2 steps: 1) only gravity load...

30 January 2021 2,772 1 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

How to check "no blast hit" sequences of NGS data output?

My environmental sample was analyzed for fungi by both DGGE (18S) and NGS (ITS2), resulted in some different names (in the top 5 of strong bands in DGGE, or most abundant sequences in NGS) between...

21 January 2021 5,310 3 View

Best way to optimize species-specific primers? Can I use a temp gradient to test a Ta where I will get target (and no non-target) amplification?

Hello, I am testing different species-specific primer sets. I have two targets (two species, but same gene target). I am trying to select the best primers -- one that will amplify the target...

18 January 2021 1,981 6 View

Can BLAST/ search by using known Domain in Transcriptome/Genome data for a gene family?

Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...

15 January 2021 2,293 3 View

How can I build a local KEGG db?

I want the info (gene name, metabolic pathway, and organism of origin) of some key identified metatranscriptome genes obtained from MG Rast (I have the sequences). The KEGG ftp site seems to...

12 January 2021 9,985 1 View

Why do I keep obtaining the same secondary PCR band along with the specific product band?

Hello everyone! I need to amplify a specific DNA fragment in order to do Sanger sequencing for the detection of a point mutation in human patients. The fragment is in an exon of my gene of...

25 December 2020 1,805 3 View