What is the better method for RNA/protein extraction: Trizol or Methanol/Chloroform?

We want to do transcriptome and phosphoproteome analyses of the same sample and have limited material. So a simultaneous extraction method for both (RNA and protein), such as Trizol or...

18 October 2020 1,708 1 View

What is the workflow for a TMT experiment on Thermo Fusion Mass Spectrometer

06 April 2016 1,509 3 View

Determine individual amplitudes of overlapping curves

I have a number of Lorentzian curves ("reference curves"), all having the same half width at half maximum, and I know each curve's location parameter. However, the reference curves are multiplied...

19 April 2012 4,017 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Can fibrosing cholestatic hepatitis in an immunocompromised patient be diagnosed without a liver biopsy?

provided that all the criteria for diagnosis has been fulfilled and alternative diagnosis are also ruled out. The patient has coagulopathy and transjugular biopsy wont be possible due to resource...

13 January 2021 8,935 4 View

In vivo mouse model - CCl4-induced liver fibrosis - problems/mortality, please help?

Hi, I have some problems with carbon tetrachloride (CCl4) -induced mouse model of liver fibrosis, and would like to ask is any of you has experience in troubleshooting this model. I run model on...

15 January 2020 5,735 6 View

In vivo mouse model - CCl4-induced liver fibrosis - problems/mortality, please help?

Hi, I have some problems with carbon tetrachloride (CCl4) -induced mouse model of liver fibrosis, and would like to ask is any of you has experience in troubleshooting this model. I run model on...

31 December 2019 9,387 3 View

Can you recommend me a fast track publication journal in the field of pulmonology/cystic fibrosis?

I am currently looking for a journal/publisher in the field of pulmonology/cystic fibrosis with the option of rapid publication. I will be very grateful for the recommendations.

11 December 2019 7,505 3 View

How can I remove unwanted "junk" from hepatic immune cells Isolation?

I am currently performing hepatic immune cells isolation from high fat diet(HFD) mice. While separating immune cells from the liver extract using Optiprep and FACS buffer gradient , in case of HFD...

10 November 2019 9,332 3 View

I'm looking for an SOP for using carbon tetrachloride to induce liver disease in mice?

I need to know what precautions need to be taken for using CCl4 in the mouse model of inducing liver fibrosis. I plan to inject the CCl4 intraperitonealy. The biosafety comittee has questions as...

13 August 2019 6,746 3 View

I'm looking for an SOP for using carbon tetrachloride to induce liver disease in mice?

I need to know what precautions need to be taken for using CCl4 in the mouse model of inducing liver fibrosis. I plan to inject the CCl4 intraperitonealy. The biosafety comittee has questions as...

07 August 2019 4,548 2 View

Does skeletal muscle stain positive for collagen?

Hi all, I did trichrome staining for dennervated rat muscle. The results demonstrated positive aniline blue staining in the muscle at about 2 to 5% of the surface area. My question is does...

05 June 2019 582 3 View

Has anyone had problems reproducing liver fibrosis using the carbon tetrachloride model in BALB/C mice??

I've been working for the past 2 years in my thesis with the CCL4 model to reproduce liver fibrosis in mice. More about the model here: The carbon tetrachloride model in mice But I've been...

27 April 2019 4,270 3 View