07 December 2021 2 8K Report

help me figure out how to answer the questions in the file I have attached, please

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Best way to preserve FACS enriched cells for downstream single nucleus RNA & ATAC seq?

I am working with a cell population that needs to be enriched by cell sorting from fresh tissue. I am wondering what the best way to preserve the cells, after sorting, for downstream single...

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What is the better method for RNA/protein extraction: Trizol or Methanol/Chloroform?

We want to do transcriptome and phosphoproteome analyses of the same sample and have limited material. So a simultaneous extraction method for both (RNA and protein), such as Trizol or...

18 October 2020 1,816 1 View

Can someone give me a w-beam guardrail or concrete barrier FE model of LS-DYNA or Hypermesh(k.file) ?

I am a postgraduate, desperately needed this model for research. Thank you！

15 October 2020 5,049 1 View

Is there a database for Cystic Fibrosis disease related genes?

I am looking for a list of genes, which are known to be affected in cystic fibrosis (differentially expressed, apart from CFTR). Is there a database available? Many thanks

25 May 2020 6,709 1 View

CFI=1, but still have degrees of freedom. Is this CFA appropriate?

In a CFA, we have very good fit indices. For example, the CFI = 1. This, however, is not a just-identified model because degrees of freedom is not 0. When I see such super excellent fit indices in...

04 March 2019 532 3 View

What is the best way to measure bacterial growth?

Im currently growing bacterial inside and outside a low pressure chamber. But, i am not sure what is the best way to Measure the growth.

24 May 2018 222 7 View

Are there any agreed-upon cutoffs for relative efficiency?

I'm conducting a monte carlo simulation and will be calculated relative efficiency. Are there any agreed-upon cutoffs for acceptable relative efficiency?

05 June 2017 7,295 1 View

How to interpret coefficients from a beta regression?

I'm interested in performing a beta regression in which the outcome is a proportion bounded between 0 and 1. I will want to interpret the coefficients of the model; however, I cannot find any...

09 March 2017 4,087 4 View

What is the workflow for a TMT experiment on Thermo Fusion Mass Spectrometer

I am searching for the best/optimal  settings for protein quantification using TMT tag and Fusion mass spectrometer (MS3 Notch method). Should I use LysC only (because of peptide labeling at...

06 April 2016 1,589 3 View

Looking for some colleagues to exchange experiences - Who has experience in applying the Welfare Quality Assessment Protocol for fattening cattle?

I'm most interested in application outsideof Europe and/or in extensive conditions

17 March 2016 3,388 4 View

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