Is there a protocol for the isolation of free fatty acids and fatty acyl-CoA/ACP (prokaryotes)? And dectection with GC-MS?

Ferdinand Kirchner @Ferdinand-Kirchner-2

25 February 2013 0 10K Report

I want to use free fatty acids or the thioesters for a pathway and want to see if I can detect the them in the wildtype. The protocols I find extract the fatty acids from phospholipids if I understand correctly, these are not available for my pathway.

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