How can I measure fibrosis in a cardiac tissue?

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Samir Ranjan Panda

Hi Saima Shakil Malik

Please refer to this YouTube video. It explains Massons Trichrome Staining quantification using ImageJ.

https://www.youtube.com/watch?v=NxNEf1XSPTk

Thanks,

Lukas Narjes

Hi! Fibrosis in cardiac tissue can be nicely visualized using WGA antibody, directly coupled to Alexa Fluor 633 for example. There is literature on that as well. Hope it helps.