I am particularly interested to study differentiation of naive T cells into iTregs.
T cells in human mononuclear cells if they are available.
Thank you Rafael, for that information. I suppose these are primary cells, right? Can these be maintained like other cell lines?
They are primary cells and can be maintained but have to be used in the experiment. They cannot be frozen to be thawed and do another experiment days later.
I know the cell line is not an adherent, it is a loosely adherent cell line. But my cells are not getting settled on the flask. they are all floating in the media. Previously when I have revived...
28 September 2020 5,765 3 View
Suppose sum of X+Y is equal to Z, where [lower limit {Z} Output-> {X,Y} So, for an example of Z= 10. 0
20 April 2020 5,541 4 View
According to Coleman's following paper (link is attached), the eigenvector corresponding to negative eigenvalue is the tangent vector to the contour at z=1, the question is how to make sure this...
22 January 2017 5,707 1 View
Please see the following link, I am actually having problem with following sentence "If a transmission line has a length greater than about 10% of a wavelength, then the line length will...
16 January 2016 6,418 5 View
This bidirectional dc dc converter can power flow in both direction one is high voltage side and other one is low voltage side. Low voltage side is current fed and high voltage side is voltage fed.
23 September 2014 7,486 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View