Most of zinc fluorescent dyes are influenced by pH change. So, is there any zinc dye pH-independent (pH~ 4 -- 11 ) and membrane permeable or impermeable? Thanks.
Dear Zihui Wang,
Attached a publication that covers the answer to your question. The following are the abstract and conclusions of the paper:
Reaction-based fluorescent sensor for investigating
mobile Zn2+ in mitochondria of healthy versus
cancerous prostate cells
Wen Chyan, Daniel Y. Zhang, Stephen J. Lippard1
, and Robert J. Radford1
SIGNIFICANCE
Mobile zinc plays important roles in mammalian physiology. Understanding the action of mobile zinc requires tools to follow it within and between live cells. Zinc-selective fluorescent probes offer a facile means for detecting such mobile zinc, but small molecules constructed to perform this task typically have an unpredictable cellular distribution. Subtle changes in chemical structure can radically alter subcellular localization. To overcome this challenge, we installed a chemical unit to direct the sensor specifically to mitochondria and discovered that tumorigenic cells lose their ability to accumulate mobile zinc within these organelles. To carry out this work, we devised a reaction-based sensor that undergoes zinc-mediated chemistry, converting a nonfluorescent molecule into one that emits brightly and avoiding undesired sequestration in endo/lysosome.
Keywords: reaction-based probe, prostate cancer, zinc biology, fluorescence microscopy
ABSTRACT
Chelatable, mobile forms of divalent zinc, Zn(II), play essential signaling roles in mammalian biology. A complex network of zinc import and transport proteins has evolved to control zinc concentration and distribution on a subcellular level. Understanding the action of mobile zinc requires tools that can detect changes in Zn(II) concentrations at discrete cellular locales. We present here a zinc-responsive, reaction-based, targetable probe based on the diacetyled form of Zinpyr-1. The compound, (6-amidoethyl)triphenylphosphonium Zinpyr-1 diacetate (DA-ZP1-TPP), is essentially nonfluorescent in the metal-free state; however, exposure to Zn(II) triggers metal-mediated hydrolysis of the acetyl groups to afford a large, rapid, and zinc-induced fluorescence response. DA-ZP1-TPP is insensitive to intracellular esterases over a 2-h period and is impervious to proton-induced turn-on. A TPP unit is appended for targeting mitochondria, as demonstrated by live cell fluorescence imaging studies. The practical utility of DA-ZP1-TPP is demonstrated by experiments revealing that, in contrast to healthy epithelial prostate cells, tumorigenic cells are unable to accumulate mobile zinc within their mitochondria.
CONCLUSION
DA-ZP1-TPP is a zinc-selective, reaction-based, fluorescent sensor that has an excellent dynamic range and pH profile, and it can be targeted to mitochondria in live cells. Acetylation of fluorescein-based zinc sensors simultaneously increases hydrophobicity and reduces the anionic character of the constructs, which in turn improves the cellular uptake and targetability of the probes. In contrast to the nonacetylated derivative, DA-ZP1-TPP has a vastly improved dynamic range and pH profile, and it can target mitochondria in live cells. DA-ZP1-TPP imaging of epithelial prostate cells revealed that tumorigenic lines lose the ability to accumulate mobile zinc within their mitochondria. Understanding mobile zinc trafficking in biology will require reagents such as DA-ZP1-TPP, which have the ability to visualize changes in free Zn2+concentrations at discrete cellular locales. By creating a mobile zinc map and tracking the accumulation and fluctuation of mobile zinc concentrations within the cell, we can begin to decipher the spatiotemporal mechanism behind the actions of mobile zinc and improve our understanding of its biology.
Hoping this will be helpful,
Rafik
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
- Biochemical Techniques
- Blotting Techniques
- Immunoblotting
- Dyes