This is a new occurrence in which samples that have GAPDH values
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.
03 March 2021 5,266 4 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...
02 March 2021 9,833 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...
01 March 2021 8,220 2 View
I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....
01 March 2021 3,325 2 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?
28 February 2021 3,864 3 View
Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...
28 February 2021 2,116 1 View
I am using my GAPDH as a housekeeping protein for my Western Blot. For all the blots I have, my GAPDH was coming out as two bands. Therefore, I am using both bands for my analysis. Has anyone...
11 February 2021 6,376 3 View
My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...
30 January 2021 1,039 3 View
I activated my membrane twice in total and hybridized it with two different antibodies. The first antibody was the specific antibody I was looking for and the second one was GAPDH. I then...
29 October 2020 5,530 6 View
Good night all. I'm using RNAi to knockdown a transcription factor from a primary cell and I need to validate by westernblot. With Ponceau marking i can see that i'm putting the same amount of...
25 August 2020 263 3 View
I have stained WT HCT116 cells with Gapdh (green fluorescence). Fixed the cells with 4% paraformaldehyde. Washed with 1X PBST. Blocked with 1% goat serum/ BSA. Incubated with primary Ab overnight....
27 July 2020 231 11 View
I transfected Huh 7.5 cell lines with vector pDG461which harbors Cas9. I performed qPCR to make sure that Cas9 is expressed in my cell lines. I used GAPDH as a reference gene in qPCR and my...
11 July 2020 7,139 11 View
Hi I have a problem with optimizing TaqMan PCR? I am getting higher CT values in housekeeping gene GAPDH but undetermined target gene values. Also to be curious, I have ran those samples on 2%...
12 March 2020 7,477 7 View
I am looking to measure NADH production at 340 nm by GAPDH (from crude protein extract) over time in a UV transparent 96-well plate. So far I am unable to detect any signal and I was wondering if...
12 February 2020 7,376 2 View
Hello folks, I am trying to cut a specific RNA using RNAse H. To do that I'm annealing an oligo which targets my RNA (annealing buffer TRIS 10mM, NaCl 50mM, 1mM EDTA) heating the mix (with...
03 February 2020 6,689 2 View
Hi all, Here is a bit of context for the question. So I took primary thymus and sliced it into 500 uM slices. I then proceeded to culture these slices for three days. After the three days, I...
01 February 2020 1,410 6 View
