WHOQOL - bref. test: how to conduct the correction?

18 January 2021 7,257 2 View

How can I differentiate the true allele from stutters and PCR artifacts in microsatellite genotyping?

Most of my microsatellite markers are dinucleotides, therefore they show stuttering. In regular stuttering is easy to identify the allele, which is the highest peak on the right, after the shorter...

26 September 2020 1,486 5 View

How could I extract residues pesticides fron sheep wool?

Hi, I want to analyse residues of Cipermetrine, ethion, amitraz, diazinon and other pesticides extracted from wool, by using HPLC/MSMS system. I would like to know the best way to extract the...

15 August 2019 9,350 2 View

How to set scale bars of images acquired from different microscopes?

I took pictures from the same tissue stained with HE and IF (two slides, same piece of tissue, different stainings) using different microscopes (both Nikon) with the same objetive (10x). However,...

21 February 2019 1,741 5 View

How to release a protein (80 kDa) from a polymeric nanoparticle?

10 November 2018 6,963 3 View

How can I collect data on social media users demographics of a specific hashtag?

I am trying to understand the relation between the demographics of Facebook users and the demographics of those using a specific hashtag in their posts.

28 November 2017 3,965 3 View

What is the best way to correlate microbial communities and environmental samples?

I am analysing microbial communities from different sources regarding cheesemaking (milk, starter culture, cheeses) and I would like to know which is the best way to correlate these microbial...

02 July 2017 3,093 3 View

Which libraries are good for signal processing in Python?

I'm learning Python and I wonder which libraries are good for signal processing?

27 October 2016 3,923 7 View

No title

10 August 2016 6,488 1 View

Alternatives for CFAR processors?

The clasical radar processors are the CA-CFAR, SO-CFAR, GO-CFAR and the OS-CFAR. I have also found good references about the AND-CFAR and OR-CFAR, the MSCA-CFAR and the IS-CFAR. I'm trying to put...

04 July 2016 3,265 5 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Can a semi-conductor material have different absorption coefficient?

Does the absorption coefficient of the semiconductor changes with the change in the structure of the semiconductor? If you have a thin film and nanowire of the same semiconductor, will the...

23 February 2021 9,799 4 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

absorption= 1-mag(S(FP1:1,FP1:1)*S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)*S(FP2:1,FP1:1)) is it correct for absorption ?

hi everyone I am simulating a unit cell structure in HFSS using Flouquet port having two modes for absorption I have used this formula:- absorption=...

17 February 2021 9,178 2 View

How to detect uv vis spectrum of nanoparticle under 300nm?

I try to make the indium nanoparticle and check the uv vis spectrum. I heard that In nanoparticle has surface plasmon resonance peak at 200~300nm. But most of solvent has absorption peak under...

14 February 2021 6,950 15 View