How can I reduce the reaction time of amylase with 4-nitrophenol alpha d maltopentaoside to form yellow color?

I have extracted, purified and lyophilized protein of insect.I use BES, HEPES and phosphate buffer.The yellow colour product is forming but after 24hrs.The reaction is carry out at 25-28 degree...

08 September 2016 1,712 7 View

How can I prepare BES buffer for amylase assay with varying pH?

please help me to prepare BES buffer for amylase assay.i have 4-nitrophenol alpha d maltopentaoside (synthetic substrate of amylase).

11 December 2015 3,309 3 View

Is there any specific method for preparation of crystals of amylase?

I have purified amylase which was extracted from insect sample.

04 May 2014 824 3 View

Is there any specific synthetic substrate for amylase of T.castaneum?

To study enzyme activity and purification of same.

11 December 2013 5,480 5 View

Is there any specific method to study the autocatalysis of enzyme?

During storage of crude extracted enzyme I observed that the number of bands are varying during gel electrophoresis.

08 September 2013 6,161 7 View

If we will increase the percentage of ammonium persulphate for polymerization of NATIVE PAGE, is there any effect on % of gel?

I already used 10% APS but there is a problem for polymerazation so instead of 10% I used 50%APS.

05 June 2013 575 8 View

What is a method to purify extracted alpha amylase from biological material?

Amylase was extracted by the centrifugation method, i.e crude amylase.

04 May 2013 9,151 2 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Can any one suggest the name of Litchi insect/predator?

Is it a type of Ambush bug or what? Thanks

14 January 2021 9,381 3 View