I have three field margin types (grassy/shrubby/tree) and each type have 5 sites. I need to calculate Shannon index for insect counts from each field margin type....

Q1. In my case how should I calculate Shannon index? Should I calculate first for each sites and giving their mean in my table(refer attachment) or neglecting the sites and taking the total count among each field margin types to calculate Shannon index.. Which one will be the perfect?

Q2. And also I need to test the statistical significance of Shannon index between various field margin types.. which test will be best to compare Shannon indices?

Please refer my attached table to get idea of what I need exactly.

Thank you very much

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