Neeta sudamrao Khillare

8 Questions 9 Answers 0 Followers

Questions related from Neeta sudamrao Khillare

How can I reduce the reaction time of amylase with 4-nitrophenol alpha d maltopentaoside to form yellow color?

I have extracted, purified and lyophilized protein of insect.I use BES, HEPES and phosphate buffer.The yellow colour product is forming but after 24hrs.The reaction is carry out at 25-28 degree...

09 September 2016 1,756 7 View

How can I prepare BES buffer for amylase assay with varying pH?

please help me to prepare BES buffer for amylase assay.i have 4-nitrophenol alpha d maltopentaoside (synthetic substrate of amylase).

12 December 2015 3,356 3 View

Is there any specific method for preparation of crystals of amylase?

I have purified amylase which was extracted from insect sample.

05 May 2014 863 3 View

Is there any specific synthetic substrate for amylase of T.castaneum?

To study enzyme activity and purification of same.

12 December 2013 5,552 5 View

Is there any specific method to study the autocatalysis of enzyme?

During storage of crude extracted enzyme I observed that the number of bands are varying during gel electrophoresis.

09 September 2013 6,207 7 View

If we will increase the percentage of ammonium persulphate for polymerization of NATIVE PAGE, is there any effect on % of gel?

I already used 10% APS but there is a problem for polymerazation so instead of 10% I used 50%APS.

06 June 2013 621 8 View

Is their any easy method to identify male and female insect of Tribolium castaneum?

I have mixed culture collected from stores.

05 May 2013 9,398 5 View

What is a method to purify extracted alpha amylase from biological material?

Amylase was extracted by the centrifugation method, i.e crude amylase.

05 May 2013 9,204 2 View