Is the production of algae to harvest oil for biodiesel done on a commercial scale?

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Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Why do I get no melt curve peak and no Ct for some of my patients but not others, in a SYBR green qPCR?

I am doing a RT-qPCR for gene expression analysis of cancer patients, and there are four different groups in the results. The first group that shows over expression of my target gene (both two...

31 May 2024 1,088 0 View

Can anyone interpret why the absorbance in alpha amylase assay get increased with increased standard (inhibitor) concentration?

Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...

26 May 2024 4,753 2 View

Anyone with phosphate flotation experience?

I attempted a phosphate (apatite) flotation, but the slurry was too thick for the impeller to work properly. I reduced the solid content to 30% for better mixing, but the conditioning still seems...

14 May 2024 4,505 4 View

Why my amino acids do not contribute in amidation reaction?

Hello everyone, recently I am trying to synthesize a dipeptide with a Coumarin derivative of Tryptophan, and the problem is it doesn't contribute in reaction at all. Tryptophan itself in the same...

15 April 2024 6,778 3 View

How to increase concentration and quality of RNA after extraction?

Hello All, I'm doing RNA extraction from cells. I tried both ways by using TRIZOL (with chloroform, and isopropanol) and RNeasy mini kit. When I use TRIZOL, the RNA concentration is high, however...

11 March 2024 9,171 8 View

How to interpret my samples' results from D1000 ScreenTape Assay?

Hello All I prepared my library to run for sequencing. I would like to check the quality of my samples through D1000 ScreenTape Assay before add to sequencer. Can anyone help me to interpret my...

10 March 2024 8,764 0 View

Can anyone help with SHSY5Y cells differntiation?

Hello all I'm working with SHSY5Y cells to differentiate them to neuronal cells. I'm following the protocol with using RA, BDNF and cAMP for cell differentiation. I tried it different times but...

19 February 2024 1,672 5 View

Why Cryo cells (freezed cells) do not attached to the flask?

Hello all I'm working with SHSY5Y cells and I always made Cryo cells from them, them thawed them and used them without problem. But, recently, after thawing, they are remain floating and not...

19 February 2024 9,305 2 View

Can someone suggest protocol to produce knock-down cells by using shRNA lentivirus particles?

Hello All, I have shRNA lentivirus particles and I want to produce self-inactivating replication incompetent viral particles in cell by myself. My purpose is to produce knock-down cells in flask...

10 January 2024 6,425 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Different ways to store biogas ?

Different Storage methods of biogas can be listed here: In India people are storing in Biogas Storage Bags made of different materials? Let us list the details here to find the best quality...

01 August 2024 635 2 View

Hello researchers, how can one get the optical density of a filamentous algae?

i am comparing the growth performances of filamentous and non filamentous forms. in a case where I can not count the cells of the filamentous algae using a hemocytometer, is it okay to do a...

30 July 2024 5,985 2 View