Is the gene responsible for the production of pectinase enzymes (polygalacturonase, pectin lyase, pectate lyase) the same?

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How to determine the presence of Protein, Lipds and Carbohydrates present in Sewage Sludge?

FTIR gives you clues regarding the functional groups, but still not so comprehensive regarding the exact matter I guess. Further, quantification is not possible. Any other methods to identify and...

31 August 2019 1,257 2 View

How to identify compounds present in ash obtained from the gasification of Sewage Sludge?

The ash remain after gasification contains a number of compounds(mainly oxides I guess). The relative amount of these compounds decides the value of Bed Agglomeration Index(BAI) and Alkali Index(AI).

28 August 2019 7,427 3 View

How to calculate the oxygen content(% by weight) of biomass having high ash content from the ultimate analysis results?

From the ultimate analysis results, the wight percentage of oxygen is normally calculated by difference(100-(C+H+N+S)). But for biomasses with high ash content, how to calculate the Oxygen?

22 August 2019 7,543 6 View

Sewage Sludge from tratment plants contains considerable amount of heavy metals. Then how gasification is a safe remedy for disposal than landfil?

Gasification reduces volume of sludge considerably but still the ash and product gas may contain the heavy metals and still it is as dangerous as direct use as fertilizer or landfill.

13 August 2019 5,395 10 View

Please help me to calculate pectinase enzyme activity (U/ml) by DNS assay?

I have also used DNS assay to calculate the activity. This is the method I used to calculate the activity If the OD 540 value is 1.04 OD, then it is equal to 0.53mg of D-galacturonic acid( from...

12 December 2016 7,472 4 View

Anyone have recently published article about Kentucky's current agriculture from socioeconomic perspective and related to sustainable agriculture?

I am looking some articles that talks about socioeconomic perspectives of Kentucky agriculture economy or simply Kentucky agriculture economy.

08 July 2016 3,783 3 View

Can you suggest me some good and conceptual papers related to concept of sustainable agriculture? and sustainable agriculture practices?

I want to be more clear about sustainable agriculture concept. Every article feature about social, economical and ecological aspects of sustainable agriculture. Is that all or there is something...

07 July 2016 1,244 8 View

What are the indicators to measure agriculture sustainability in farm level?

I want to run logit regression model between different sustainable practices and socioeconomic factors affecting adaption of different sustainable practices. I think it is hard and illogical to...

01 March 2016 5,405 7 View

Where can I find reference data for land cover classification of Nepal?

I want to do accuracy assessment of land cover classification of Kaski, Nepal that I have done. But, due to lack of reference data, I am unable to do so. How Can I find that data? Selecting random...

23 April 2015 8,945 3 View

How do I calculate the specific activity of exo polygalacturonase(pectinase)?

Please help me to calculate the Specific activity of exo-polygalacturonase (pectinase) from bacillus culture. Enzyme assay was done by DNS method

21 September 2014 6,778 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View