Kavuthodi Bijesh

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Questions related from Kavuthodi Bijesh

Please help me to calculate pectinase enzyme activity (U/ml) by DNS assay?

I have also used DNS assay to calculate the activity. This is the method I used to calculate the activity If the OD 540 value is 1.04 OD, then it is equal to 0.53mg of D-galacturonic acid( from...

13 December 2016 7,441 4 View

Is the gene responsible for the production of pectinase enzymes (polygalacturonase, pectin lyase, pectate lyase) the same?

Through enzyme assay, I am getting result for Polygalacturonase (DNS assay) , Pectin lyase, pectate lyase (TBA assay) and pectin esterase . Is the gene responsible for the production of these...

01 May 2015 7,138 2 View

How do I calculate the specific activity of exo polygalacturonase(pectinase)?

Please help me to calculate the Specific activity of exo-polygalacturonase (pectinase) from bacillus culture. Enzyme assay was done by DNS method

22 September 2014 6,761 4 View

How can I estimate the total pectin content from a agro product/waste?

11 June 2014 7,722 2 View

Could anyone provide me with information regarding the gene structure of Bacillus subtilis?

Bacillus subtilis - responsible for pectinase (Exo-Polygalacturonase) enzyme production.

02 April 2014 2,715 1 View

For the pectate lyase assay, is it necessary to use Polygalacturonic acid as a substrate? Can I use pectin (sigma) as a substrate?

see above

27 March 2014 8,579 3 View

Could someone explain the complete procedure for pectin lyase assay?

I am interested in the procedure for my research work.

25 September 2013 9,070 3 View