Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Does SSMPCA toolbox (scanvp) provide a module to perform the bootstrapping procedure to estimate the reliability of the covariance pattern?

I'm learning to perform the multivariate covariance analysis with SSMPCA toolbox (scanvp/ssmpca, www.feinsteinneuroscience.org). The step-by-step procedure to obtain covariance pattern and...

08 February 2021 2,383 1 View

Has anybody used the Chicken anti-mCherry antibody (ab205402) in immunohistochemistry?

I'm looking for a primary antibody to detect tdTomato in spinal cord slices of mutant mice. I know that the most widely used antibody for this purpose is the mouse anti-RFP from Rockland, but I...

02 February 2021 5,515 3 View

I am culturing murine bone marrow derived mesenchymal stromal cells, what are these cells I am observing?

Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...

24 January 2021 7,223 3 View

I am inducing ostegenic differentiation in murine bone marrow derived mesenchymal stromal cells, what are these cells I am observing?

Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...

24 January 2021 3,678 3 View

How can I create a custom corpus for text mining with nltk/spacy?

Hi, I am quite a newbie with python, and I need to run some text mining analysis on 100+ literary texts in German, which I have stored as individual txt files in a folder. They are with the scheme...

20 September 2020 4,762 3 View

Which rapid in-line/on-line method could be used to ensure extraction yield for instant coffe and how to calibrate it on chemometric methods?

There is a need to ensure extraction yield of the soluble coffee process, thus we need a method to analyze carbohydrate degradation products produced after hydrolisys of polysaccharides due to...

11 March 2020 3,308 2 View

How high can be the solar radiation measured outdoor in Australia?

Dear all, I am using a weather station to measure some environmental parameter in the city of Sydney, pedestrian level. In summertime, the global solar irradiation (horizontal) returned by the...

08 March 2020 7,599 6 View

Anyone having problems with low DNA concentrations after RNase A treatment (during DNA extraction)?

I am currently testing the GeneAid DNA extraction kit on abalone (Haliotis iris) attachment muscle tissue samples and it was working pretty fine, until I added the RNase A treatment (as stated in...

26 January 2020 3,019 1 View

HPLC for AAV purification?

Hello, I'm looking into buying a new HPLC machine for purification of AAV preps. Has anyone had a good (or bad) experience with a particular model? Thank you.

22 January 2020 4,024 6 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

No title

23 February 2021 4,014 3 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

22 February 2021 6,569 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

21 February 2021 7,561 4 View

What properties of water shall be analyzed and evaluated to test the effectiveness of activated carbon?

I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?

20 February 2021 9,422 8 View