10X Buffer with MgCl2! What is the use of tagging, if the buffer doesn’t work without any addition of extra MgCl2?

Can anyone please tell me if you tried PCR run without the need of adding an extra aliquot of MgCl2 and still have got the amplification? I tried with two suppliers but they didn’t work out for...

12 December 2019 2,477 2 View