03 March 2014 1 4K Report

I'm just wondering if this kind of staining is linear with the concentration of DNA.

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Can you help me with pharmacopoeia procedure 3.2.1 on arsenic determination?

Hi to all I am writing you in order to get more information about the procedure 3.2.1 on Arsenic determination in glass containers for pharmaceutical use. In the monograph, at the paragraph...

05 June 2018 1,377 3 View

How do you overcome the following issues with S and Os ICP-OES?

hi to all, i'm experiencing several issues with detection of elements such as sulfur and osmium at icp-oes. in particular, i work with an Agilent ICP, and at each analysis, i have to analyse these...

08 September 2017 5,505 3 View

Is it possible to infere from isoelectric point the superficial pH?

hi to all, i would like to estimate the superficial pH on spherical particles having dimension of nanometers. since the surface is regular and omogeneusly coated, i was wandering if could i infere...

02 March 2016 9,046 2 View

Can amicon ultra devices be used at 4°C?

hi to all, I need to use amicon ultra filtration devices at low Temperatures in order to purify a protein sensitive to this parameter. can i use amicon devices in a centrifugue refrigerated at 4°C?

02 March 2016 8,307 4 View

How can I stop protein induction?

hi to all, i was wandering if it is enough to pellet cells (e.coli) from the protein induction medium containing iptg and resuspend them in a new medium without inductor to stop the protein...

01 February 2016 3,643 3 View

Is it possible to co-trasform E.Coli bl21 de3 gold with two iptg inducible plasmids?

hi to all, i was wandering if it is possible to transform bl21 de3 cells with two plasmids. the aim is to espress 2 proteins at once. the two plasmids are both iptg inducible, and ampicillin...

01 February 2016 3,119 8 View

Is it strictly necessary the chloramphenicol in LB agar plates for pLysS cells, or the ampicillin is enough?

If I add chloramphenicol only in the overnight liquid culture, is ok? should the chloramphenicol be present also during the induction of the protein? thank you

31 December 2015 1,255 3 View

Could the OD_600 of a bacterial culture be a clue of protein expression?

hi to all, i have noticed that if i incubate two parallel bacterial cultures in the same conditions ( one where i add IPTG for protein induction and another in which I don't add anything) the OD...

31 December 2015 1,080 7 View

Can glucose polymers be used by E.Coli as carbon Source?

Hi to all, I'm wondering if glucose polymers such cyclodextrins, glycogen and more complex polymers, are metabolized by E.Coli. Thank you

09 October 2015 2,798 4 View

How can I dock a 4Fe-4S cluster into a protein?

hi to all, I'm trying to dock a 4Fe-4S cluster into a protein using the Haddock web-tool. I suppose that the protein I'm using coordinates the cluster by means of specific residues, that I have...

05 June 2015 5,973 0 View

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