Can amicon ultra devices be used at 4°C?

More Antonio Rosato's questions See All

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Filippo Prischi

Yes, I always concentrate at 4C

Antonio Rosato

is it slower? respect to room temperature?

Abdulhadi Albaser

As a rule always work at 4C to avoid proteolysis which may occurred at room temp. There are several sizes with different cut off so choose the one suits the molecular weight of your protein, they mainly used for concentrating proteins.

Good luck

the increase in density of the buffer does not affect much the speed of concentration.