For peptide treatment in cell culture, which should be the preferred method - with serum or without?
Does peptide bind to albumin in serum?
Does serum (FBS) contain any proteases?
If we will treat with serum free, then we have to change the media after incubation up to 4-8 hours with complete media.
But we need 24 hour of treatment.
It's hard to say a priori, given that you haven't stated what cells or anything about the peptide, but in general serum free conditions are best. Serum does contain proteases and albumin is quite "sticky" so it can potentially bind your drug. You'd have to test it to be sure.
You're best bet is probably a defined growth medium that does not contain serum or has a greatly reduced serum amount.
To Peter
Can you mention the incubation period of treatment with Serum free (SF) media.In our lab some people do 4 hours of treatment with peptide in SF followed by change the media and do incubation for 24 hour.but my question is whether the incubation is actual 24 hour ? or 4 hour?
Again at some concentraion (effective-inside the cell) cause only cellular arrest but not cytotoxic. If incubated with peptide for 24hours (complete) might be resulted into cell death.What If SF media will be used to treat and again changed with fresh complete media after 4 hours ? cell death or cell regrowth?
It's impossible for me to say, since you haven't even revealed what cells you're using.
In general, I think you simply need to do the proper vehicle controls. So if you're using serum free media to deliver the peptide, have some control cells in the same media, then compare at the various time points. You can then add complete media and further follow in time.
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