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Questions related from Saswat Mohapatra
Need a simple mathematical solution to quantify the NO in side the cells using DAF-FM as NO detector. I have used one NO delivering agent to increase the NO in cells but could not able to...
28 March 2017 6,935 4 View
Need a protocol to define ROS generation in different cellular compartments such as cytoplasm and mitochondria. Also need suggestion of any particular inhibitor of ROS for respective compartments
23 March 2017 679 3 View
I came across a sound number of techniques regarding analytical approach on cancer stem cells or cancer initiating cells (CSC). But I could not able to find out any standardized method to increase...
15 August 2016 1,468 6 View
The peptide I am working with is showing cytotoxicity in cancer cells. Thus, I wonder whether this is due to the cationic surfactant nature of the peptide as it has many cationic groups available...
28 April 2016 3,294 14 View
I need to work on in vitro tumor model that will some what similar to the natural tumor. In the model to develop, macrophage (M2 phage) is very crucial including other cells that generally reside...
12 August 2015 4,550 5 View
Can any body suggest methods to analyse the biniding site for any molecule.i have already some molecules having interaction with microtubule but now I need to know the exact binding site on the...
25 December 2014 5,934 2 View
To understand the action of some microtubule target agents. Can anybody help me with a good protocol or work design to analyse depolymerisation of microtubule
17 August 2014 1,342 3 View
For peptide treatment in cell culture, which should be the preferred method - with serum or without? Does peptide bind to albumin in serum? Does serum (FBS) contain any proteases? If we will treat...
17 August 2014 4,397 4 View
Can anybody help me regarding a small molecular screening tool to analyse different lead molecules and how to designing a new one
17 August 2014 9,713 2 View
We have tried to localize the p53 and p21 in MCF-7 cells after treatment of drug. We found that p53 located around the nucleus (hoechst stained) but very rare inside signals. Again in negative...
11 August 2014 1,693 6 View
Because one of our drug is fluorescent active we are not able to detect ROS through florescent detection method.
30 July 2014 3,206 6 View
One of the drug we found to affect microtubule still not able to do any cytostatic effect.What could be the interpretation?
30 July 2014 3,659 3 View
Hi,can any body tell me the easiest cell culture protocol to calculate metaphase / anaphase ratio?
06 February 2014 5,463 2 View
I've found that one of my drugs (peptide) causing shrinkage of nucleus and microtubule destabilization. I have also found that the drug is causing apoptosis of cell via p53 and p21 pathway etc....
17 January 2014 3,340 3 View
The Golden Ratio is generally used in mathematics and arts science based on Fibonacci number. Definitely we have used this technique after the great mathematician, i.e. nature. You can see that...
19 July 2013 7,129 3 View
I need to isolate RNA from spermatozoa of Bovine.I have tried it many time till 260/280 ratio came on an avg of 1.5.The RT-PCv Rreaction taking 550ng of RNA also shows lesser band for house...
09 January 2013 6,439 0 View
