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Questions related from Saswat Mohapatra
Need a simple mathematical solution to quantify the NO in side the cells using DAF-FM as NO detector. I have used one NO delivering agent to increase the NO in cells but could not able to...
28 March 2017 6,876 4 View
Need a protocol to define ROS generation in different cellular compartments such as cytoplasm and mitochondria. Also need suggestion of any particular inhibitor of ROS for respective compartments
23 March 2017 603 3 View
I came across a sound number of techniques regarding analytical approach on cancer stem cells or cancer initiating cells (CSC). But I could not able to find out any standardized method to increase...
15 August 2016 1,386 6 View
To understand the action of some microtubule target agents. Can anybody help me with a good protocol or work design to analyse depolymerisation of microtubule
17 August 2014 1,253 3 View
For peptide treatment in cell culture, which should be the preferred method - with serum or without? Does peptide bind to albumin in serum? Does serum (FBS) contain any proteases? If we will treat...
17 August 2014 4,335 4 View
Can anybody help me regarding a small molecular screening tool to analyse different lead molecules and how to designing a new one
17 August 2014 9,668 2 View
We have tried to localize the p53 and p21 in MCF-7 cells after treatment of drug. We found that p53 located around the nucleus (hoechst stained) but very rare inside signals. Again in negative...
11 August 2014 1,643 6 View
Hi,can any body tell me the easiest cell culture protocol to calculate metaphase / anaphase ratio?
06 February 2014 5,396 2 View
The Golden Ratio is generally used in mathematics and arts science based on Fibonacci number. Definitely we have used this technique after the great mathematician, i.e. nature. You can see that...
19 July 2013 7,062 3 View
