Saswat Mohapatra

11 Questions 11 Answers 0 Followers

Questions related from Saswat Mohapatra

How to quantify NO inside the cell with DAF-FM?

Need a simple mathematical solution to quantify the NO in side the cells using DAF-FM as NO detector. I have used one NO delivering agent to increase the NO in cells but could not able to...

28 March 2017 6,882 4 View

How to differentiate cytoplasm to mitochondrial ROS generation ?

Need a protocol to define ROS generation in different cellular compartments such as cytoplasm and mitochondria. Also need suggestion of any particular inhibitor of ROS for respective compartments

23 March 2017 611 3 View

Is there any technique available to enrich cancer stem cell population?

I came across a sound number of techniques regarding analytical approach on cancer stem cells or cancer initiating cells (CSC). But I could not able to find out any standardized method to increase...

15 August 2016 1,400 6 View

What will be the best method to find out the tubulin binding site for any molecule?

Can any body suggest methods to analyse the biniding site for any molecule.i have already some molecules having interaction with microtubule but now I need to know the exact binding site on the...

25 December 2014 5,885 2 View

Can anybody suggest an in-vitro method to analyse the microtubule depolymerisation?

To understand the action of some microtubule target agents. Can anybody help me with a good protocol or work design to analyse depolymerisation of microtubule

17 August 2014 1,270 3 View

Is serum free media required for peptide drug treatment?

For peptide treatment in cell culture, which should be the preferred method - with serum or without? Does peptide bind to albumin in serum? Does serum (FBS) contain any proteases? If we will treat...

17 August 2014 4,343 4 View

Which online tool will I use for small molecule screening?

Can anybody help me regarding a small molecular screening tool to analyse different lead molecules and how to designing a new one

17 August 2014 9,676 2 View

Can anyone help regarding immuno-localisation of p53 and p21?

We have tried to localize the p53 and p21 in MCF-7 cells after treatment of drug. We found that p53 located around the nucleus (hoechst stained) but very rare inside signals. Again in negative...

11 August 2014 1,651 6 View

Can any body suggest to me any good method to detect ROS in cancer cells-colorimetric analysis?

Because one of our drug is fluorescent active we are not able to detect ROS through florescent detection method.

30 July 2014 3,157 6 View

Mitotic index calculation protocol?

Hi,can any body tell me the easiest cell culture protocol to calculate metaphase / anaphase ratio?

06 February 2014 5,405 2 View

Can we use the Golden Ratio in genome studies?

The Golden Ratio is generally used in mathematics and arts science based on Fibonacci number. Definitely we have used this technique after the great mathematician, i.e. nature. You can see that...

19 July 2013 7,072 3 View