Sephadex G75 is definitely re-usable (unless you load something very dirty and clog your column).
With DEAE is it little more complicated. Being it a weak anion exchanger, you should regenerate the column (however, it is re-usable as well), but I've used it without regeneration and it worked.
Sephadex G75 is definitely re-usable (unless you load something very dirty and clog your column).
With DEAE is it little more complicated. Being it a weak anion exchanger, you should regenerate the column (however, it is re-usable as well), but I've used it without regeneration and it worked.
Both are re-usable. Although as noted you should regenerate DEAE before re-use. We do this routinely in the lab with no problems. We re-generate with a 2 M NaCl solution in the start buffer at 10ml/min and monitor with UV.
As others already said, DEAE-cellulose columns are reusable. However, because the material is fragile, it tends to break into tiny bits (fines) that can clog the column. Also, it cannot stand up to any pressure and will compress until it becomes impermeable. Therefore, it must be treated carefully to keep it in a useful state.
Sigma-Aldrich gives instructions on how to regenerate DEAE-cellulose. https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/d3764pis.pdf To keep from getting those "fines" that will clog the column, use very gentle suction. In addition, I suggest that before you pour the column, do not use suction for the last wash. Instead, suspend the resin in a tall beaker and watch while the resin settles. When most of it has settled but there are still fine particles in the liquid above the packed resin, decant very carefully. That should help to reduce the fine particles. This also means that you should start with a bit more resin than you need, perhaps by adding new resin at some point in the process. Otherwise your column will get shorter after each regeneration.
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Is it necessary to wash DEAE column with NaOH every time you use or just when you observe a decline in the column capacity?
I don't think it is necessary to wash with NaOH every time, especially if you are going to repeat the same purification. You can run a high salt concentration through to elute everything that is going to elute. If you want to be absolutely sure that there will be no carryover of one protein into the purification of another, NaOH is a good way to assure this. For example, suppose you purify a protease with the column. You would want to be very sure that any protease that remained in the column was destroyed before you purified something else.
If you are using DEAE-cellulose as the resin, you probably won't want to reuse the column more than one or two times because the cellulose will break up into fine particles and the column will clog.
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