I've gotten reliable results with SYBR-green based assays for miRNAs that are mid-high in abundance. I used a poly A tailing method to make the cDNA which had a universal primer as a "forward" sequence. 

However, if you can afford it and don't know how primer design works, I'd say Taqman is a safer way. Taqman will also be more sensitive to differentiating between closely-related family of miRNAs that are nearly identical in sequence. 

So for the easy stuff, go with custom primers + SYBR green, hard stuff Taqman. 

Hello, TaqMan is always more sensitive and specific than CYBR green but having more cost.

Hi,

In my opinion Taqman probes are more sensitive and reliable but a costly affair!!!

Hi Noha,

Well both will give you results, however Taqman qPCR provides you with more Specificity and Reproducibility. Please check the following link: http://www.thermofisher.com/de/de/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center/real-time-pcr-basics/taqman-vs-sybr-chemistry-real-time-pcr.html

If you needed a help in anything, please let me know. Best of luck to you!

Alaa