Weiler,E.W.,J.Stockigt and H.Zenk, 1981.Radioimmunoassy for quantitative determination of scopolamine.Phytovhemistry 20,2009-2016
Hi, I usually use sucrose lysis buffer ( 0.6 M sucrose, 0.02 M Tris base, 0.01 M MgCl2 and 2% triton) for RBCs lysis in DNA extraction. After initial RBCs lysis and washing, a pellet of WBCs is...
10 July 2018 5,442 2 View
For prediction of gene expression mostly cellular mRNA quantitation is used. I want to ask if the circulating mRNA could reflect the cellular mRNA level and thus used instead in other words if...
08 September 2016 2,717 4 View
What's ur opinion regarding using SYBR green qPCR master mixes for miRNA quantification? What is the better SYBR green or TaqMan? And if I worked with SYBR Green, the results could be reliable???
07 August 2016 7,316 4 View
if i want to measure some microRNAs from the plasma of a transfusion dependant patient, what is the minimum period after the last blood transfusion in order to take a representative blood sample
06 April 2016 5,835 4 View
03 April 2016 5,059 5 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...
01 March 2021 5,035 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...
28 February 2021 7,397 8 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...
26 February 2021 956 13 View
I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...
25 February 2021 8,039 1 View