Weiler,E.W.,J.Stockigt and H.Zenk, 1981.Radioimmunoassy for quantitative determination of scopolamine.Phytovhemistry 20,2009-2016
Hi, I usually use sucrose lysis buffer ( 0.6 M sucrose, 0.02 M Tris base, 0.01 M MgCl2 and 2% triton) for RBCs lysis in DNA extraction. After initial RBCs lysis and washing, a pellet of WBCs is...
10 July 2018 5,572 2 View
For prediction of gene expression mostly cellular mRNA quantitation is used. I want to ask if the circulating mRNA could reflect the cellular mRNA level and thus used instead in other words if...
08 September 2016 2,812 4 View
What's ur opinion regarding using SYBR green qPCR master mixes for miRNA quantification? What is the better SYBR green or TaqMan? And if I worked with SYBR Green, the results could be reliable???
07 August 2016 7,398 4 View
if i want to measure some microRNAs from the plasma of a transfusion dependant patient, what is the minimum period after the last blood transfusion in order to take a representative blood sample
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03 April 2016 5,137 5 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.
25 July 2024 6,505 2 View
Autonomous Language Learning can be implemented from primary to tertiary education. Practioners and students report on its effectiveness, however, there appears to be little quantitative or...
20 July 2024 2,592 2 View
Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...
20 July 2024 2,033 7 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
What are research philosophy and paradigm and importance of quantitative research in philosophy?
10 July 2024 585 6 View
I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...
09 July 2024 4,857 2 View
Pir Hassan Ali Shah post a question: How to do quantitative and qualitative data analysis after data collection? Give and review the answer in brief. All family members, friends, associates,...
08 July 2024 395 8 View
I'm analyzing abundances from various ecological surveys. So data was collected across multiple independent years from three sites within each year. The years fall under two temperature...
26 June 2024 2,798 2 View
I am currently attending MA in Community Development at Myanmar Institute of Theology, with my thesis scheduled for submission next semester. My first research used quantitative methods, and I...
26 June 2024 1,247 2 View