Sunil Kokane

4 Questions 13 Answers 0 Followers

Questions related from Sunil Kokane

Can anyone suggest me how to do conjugation of colloidal Gold nano particle with Polyclonal antibody?

Please give me protocol

01 January 2019 6,804 4 View

Anybody can help me for quantification of pathogen titer (copy number) ....?

I want to calculate the copy number of pathogen from from standard universal regression equation Y= 13.82-0.2866X. where, Y= is the estimated log concentration of templates X= is the qPCR Ct...

04 April 2017 3,680 0 View

Can anybody guide me about nested PCR?

I have a very critical problem related to my above mentioned work. Outer primer specific to this pathogen having amplicon size of 1.8 kb and two pair of inner primer have 1.2 as well 0.8 kb. But...

11 November 2016 5,340 8 View

Any one could help me for optimization of Real Time PCR withTaqman probe ?

Hello, I am working on Real Time PCR withTaqman probe, and used 1 ul DNA (26ng/ul)but i got very late Ct value. how can I optimize for early Ct value? either by increasing amount of DNA or by...

08 August 2016 6,998 7 View