8 Questions 11 Answers 0 Followers
Questions related from Alaa Ibrahim
Hello all, I'd like to get further insights on the disadvantages of using knockout mice/global knockout. Also, how can the one further study the effect of this gene/s' knockout. Thanks in...
07 July 2018 5,412 3 View
Dear all, I am wondering what is the best way to compare gene expressions in Taqman qPCR. Is it the 2^ddCT values or the 2^dCT? I want to compare the relative expression of different assays in...
04 April 2018 10,010 4 View
A question for those who are familiar with the German educational system: Which title in your opinion is more internationally recognized- while getting a doctoral degree in biology/medicine: : 1....
03 March 2018 2,010 5 View
When I did the qPCR experiment using the TLR4 assay on spinal cord RNA of TLR4 KO mouse, the TLR4 mRNA was measurable. I did the genotyping of this tissue sample, and it was a homozygous clean KO...
12 December 2017 2,617 3 View
Hello everyone! I kindly need a suggestion for a free- user friendly online bibliography and citation tool please? Other than Endnote as it's super expensive for me? Compatibility with Mac is...
11 November 2016 3,660 15 View
Hi all! Can a mouse whole vertebral column be stored in 4% PFA for longer than 24 hours, but shorter than one week? I plan to put the vertebral columns of my mice in PFA on a Friday and do sucrose...
09 September 2016 9,412 10 View
Dear all, Your help is needed here: We have a list of 700 proteins: is it possible to find out, which ones are relevant for pain and inflammation without having to check them manually? Something...
04 April 2016 942 3 View
Hi, To extract RNA properly out of a whole Skin sample (mouse), how can I homogenize the sample? would the ceramic beads be enough for that? and later, which is better, TriFast or Trizol? Thanks...
11 November 2015 3,495 4 View