Alaa Ibrahim

8 Questions 11 Answers 0 Followers

Questions related from Alaa Ibrahim

What are the disadvantages of using knockout mice?

Hello all, I'd like to get further insights on the disadvantages of using knockout mice/global knockout. Also, how can the one further study the effect of this gene/s' knockout. Thanks in...

07 July 2018 5,409 3 View

Which is a better formula : 2^ddCT or 2^dCT in comparing gene expression in 2 different mice strains?

Dear all, I am wondering what is the best way to compare gene expressions in Taqman qPCR. Is it the 2^ddCT values or the 2^dCT? I want to compare the relative expression of different assays in...

04 April 2018 10,008 4 View

To graduate with a PhD or Dr.rer.nat?

A question for those who are familiar with the German educational system: Which title in your opinion is more internationally recognized- while getting a doctoral degree in biology/medicine: : 1....

03 March 2018 2,008 5 View

Why is the gene expression in TLR4 knock-out mice only reduced but not completely absent when the targeted gene was knocked out in TLR4 KO mice?

When I did the qPCR experiment using the TLR4 assay on spinal cord RNA of TLR4 KO mouse, the TLR4 mRNA was measurable. I did the genotyping of this tissue sample, and it was a homozygous clean KO...

12 December 2017 2,614 3 View

A suggestion for a free citation tool please?

Hello everyone! I kindly need a suggestion for a free- user friendly online bibliography and citation tool please? Other than Endnote as it's super expensive for me? Compatibility with Mac is...

11 November 2016 3,658 15 View

How long a tissue fixation in 4%PFA can last before damaging the SC?

Hi all! Can a mouse whole vertebral column be stored in 4% PFA for longer than 24 hours, but shorter than one week? I plan to put the vertebral columns of my mice in PFA on a Friday and do sucrose...

09 September 2016 9,410 10 View

Search engine for proteins and their relevant pathways?

Dear all, Your help is needed here: We have a list of 700 proteins: is it possible to find out, which ones are relevant for pain and inflammation without having to check them manually? Something...

04 April 2016 940 3 View

Any advice on RNA isolation of skin sample for rt-PCR?

Hi, To extract RNA properly out of a whole Skin sample (mouse), how can I homogenize the sample? would the ceramic beads be enough for that? and later, which is better, TriFast or Trizol? Thanks...

11 November 2015 3,493 4 View