I am trying to measure microglia activation in response to an insult with qPCR. I have used Iba1 and found no differences in the expression between my groups at different timepoints. I am using a developmental model where the insult occurs at postnatal day 10. We have found increase in MhC II+ cells in the same model using flow cytometry which show that there is microglia activation... now I am trying to look for other markers of microglia activation with qPCR. As there was no difference with Iba1, I was wondering if maybe there was another marker that could show differences since during development microglia can present different populations. I have seen a talk about differences in microglia population measured by Iba1+ and P2Y12 + cells. So, I am wondering if measuring P2Y12 expression could be an interesting approach. Any ideas?