Does anyone know if the intruder animal (C57) must be kept 24 hours overnight in a separate compartment after the attack session with each resident day-by-day until the last 10º days for visual contact, or if this could be only on the first day of defeat stress? Each day I used to change the animal resident to attack my stressor intruder. I noticed that after five days of defeat, the resident didn't want to attack the intruder. Could anyone help me with some technical questions?