SPSS - how to create new variable include and excluding cases based on another variable?

Hi there, I have been searching and searching and cannot find an answer to this problem... I have a variable (Ref_Accepted_Comb) for the number of referrals accepted with values of (1=Yes, 2=No,...

08 February 2021 3,479 3 View

Does maternal potentiation work?

Has anybody been able to replicate finding of papers that show increased vocalization in mice pups after a brief period of interaction with their dam, also called maternal potentiation? We have...

11 January 2021 558 2 View

Anybody know how can I analyze differences between groups vocalization syntax using the transition probability between syllables?

I have been using a MATLAB script called DeepSqueak to analyze vocal communication in mice. One of the outcomes of this analysis is the transition probability between the different syllables that...

09 December 2020 1,525 1 View

Anyone with experience conducting proximity ligation assay (PLA) in mouse heart tissue ?

I was wondering if anyone had any experience doing proximity ligation assays (PLA) using mouse whole heart (ventricular) tissue (in any state)?

29 November 2020 9,103 1 View

How to extract/remove ethanol from kava tincture?

Producing Kava tincture using different alcohol concentrstion as solvent, soaked for 3 weeks filter off the kava paste and left with mixture of kavalactones and ethanol..need a method to remove...

14 June 2020 5,104 1 View

Can you have one variable load onto two factors in a CFA, prior to SEM?

I am running a SEM, and I have 6 variables which will load onto two factors (positive and negative mental health). However, this model does not meet a good model fit. When I allow depression to...

19 May 2020 8,286 3 View

When does someone use corrected fin length and the actual fin length in heat transfer problems?

In some instances the extended length of a fin is used but then in other instances the normal fin length is used in extended surface heat transfer problems.

23 March 2020 7,553 4 View

How will I calculate the surface temperature of a HDPE pipe that is exposed to the sun?

I need to calculate the final temperature of a pipe that's been lying in the sun for the day, however, I am unsure how to do this. I know how to calculate the heat transfer through the pipe wall...

19 January 2020 1,259 10 View

Mouse islet isolation without pancreas perfusion?

Is there a current/reliable way to isolate mouse islets (keeping their morphology and viability for culturing in vitro) without perfusing the pancreas through the bile duct?

14 September 2019 3,577 1 View

What is the best software to do detrended correspondence analysis of diatoms in a swamp?

I tried PAST3 but I want to deal with other software, preferably free software

30 April 2019 5,210 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View