Danielle Santana Coelho

9 Questions 8 Answers 0 Followers

Questions related from Danielle Santana Coelho

What should I use to homogenize samples for measurements of Oxidative Stress?

I bought a lipid peroxidation kit from Sigma and I want to get kits to measure nitrates, superoxide dismutase, catalase, and glutathione. Each one of those kits comes with a different lysis...

08 May 2024 4,977 2 View

Can I correlate the expression of different targets in the same sample quantified by qPCR?

I did an experiment where I administrated LPS in WT and KO animals. I wanted to know if the KOs have a different immune response than WTs. As targets I used several cytokines and complement system...

23 March 2023 2,784 4 View

Can you suggest good antibodies for western blot to measure the complement proteins C1q, C3 and C5 in mice brain tissues?

Hello, I am starting to work with the complement system and I have been told the the antibodies available are not the best. Anybody with experience using Ab to measure complement system protein in...

28 April 2022 8,856 0 View

Is P2Y12 expression a good marker for microglia activation in whole brain?

I am trying to measure microglia activation in response to an insult with qPCR. I have used Iba1 and found no differences in the expression between my groups at different timepoints. I am using a...

20 January 2022 5,945 2 View

Microglia activation with the viral mimetic Poly IC. Does it happen in vivo?

Hello, I am trying to find a reference for a dose of Poly IC that causes activation of microglia in mice. But all the studies I've found are in vitro. Does anybody know if there is any study...

28 May 2021 7,374 4 View

Does maternal potentiation work?

Has anybody been able to replicate finding of papers that show increased vocalization in mice pups after a brief period of interaction with their dam, also called maternal potentiation? We have...

12 January 2021 928 2 View

Anybody know how can I analyze differences between groups vocalization syntax using the transition probability between syllables?

I have been using a MATLAB script called DeepSqueak to analyze vocal communication in mice. One of the outcomes of this analysis is the transition probability between the different syllables that...

10 December 2020 1,897 1 View

My control isotype antibodies seems to have an effect when compared to my saline controls. What to do now?

I have an experiment where I am trying to see if IL-6 alters the expression of some enzymes. In order to test that I used a neutralizing antibody for IL-6 and an Isotype control. After doing the...

10 November 2019 7,077 1 View

How to do RNA extraction from cells in RNA later?

I got some samples from another lab where there are cells frozen in RNAlater. Now I am trying to extract the RNA and the yield is really low. I first used the PureLink RNA mini Link. And it didnt...

22 February 2019 8,973 3 View