9 Questions 8 Answers 0 Followers
Questions related from Danielle Santana Coelho
I bought a lipid peroxidation kit from Sigma and I want to get kits to measure nitrates, superoxide dismutase, catalase, and glutathione. Each one of those kits comes with a different lysis...
08 May 2024 4,961 2 View
I did an experiment where I administrated LPS in WT and KO animals. I wanted to know if the KOs have a different immune response than WTs. As targets I used several cytokines and complement system...
23 March 2023 2,774 4 View
Hello, I am starting to work with the complement system and I have been told the the antibodies available are not the best. Anybody with experience using Ab to measure complement system protein in...
28 April 2022 8,845 0 View
I am trying to measure microglia activation in response to an insult with qPCR. I have used Iba1 and found no differences in the expression between my groups at different timepoints. I am using a...
20 January 2022 5,935 2 View
Hello, I am trying to find a reference for a dose of Poly IC that causes activation of microglia in mice. But all the studies I've found are in vitro. Does anybody know if there is any study...
28 May 2021 7,361 4 View
Has anybody been able to replicate finding of papers that show increased vocalization in mice pups after a brief period of interaction with their dam, also called maternal potentiation? We have...
12 January 2021 918 2 View
I have been using a MATLAB script called DeepSqueak to analyze vocal communication in mice. One of the outcomes of this analysis is the transition probability between the different syllables that...
10 December 2020 1,890 1 View
I have an experiment where I am trying to see if IL-6 alters the expression of some enzymes. In order to test that I used a neutralizing antibody for IL-6 and an Isotype control. After doing the...
10 November 2019 7,065 1 View
I got some samples from another lab where there are cells frozen in RNAlater. Now I am trying to extract the RNA and the yield is really low. I first used the PureLink RNA mini Link. And it didnt...
22 February 2019 8,964 3 View