Is MTT assay work in saccharomyces cerevisiae to check cells viability?

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Arda Sever

Dear Shambhu,

Yes, you can use MTT assay in S. cerevisiae. Don't get me wrong, i am happy to help but, with a quick research on pubmed or even google, you are able to find an answer to this question. Waiting for you question to get answered might waste your time.

Best wishes.

Mohammad Tariq Ali Khan

MTT is based on succinate dehydrogenase present in mitochondria of cells. Upon addition of MTT reagent this is convreted into formazon crystal.

If S. cerevisiae has this enzyme. Yes you can use MTT no problem.

Take help from protocol.

Shambhu Yadav

Dear Arda

Thank you for suggestion .

Actually, I did googling and pubmed search but I did not get proper answer.

Dear Tariq

Thank you for sharing MTT assay protocol.

Ismaila Yusuf

Hi Shambhu Yadav,

Did your MTT assay work in S. cerevisiae?