Though miRNAs affected by blood transfusions and the clinical implications of these changes are not well understood, blood transfusions may alter miRNA levels in certain cell types, such as red blood cells, and platelet-derived extracellular vesicles; mainly regulate gene expression.

And yes, it is possible to collect samples from patients who have received a blood transfusion to measure microRNA (miRNA) levels.

Md. Niaz Morshed I want to collect Blood from patients with hematological malignancies at different stages of the disease. After that, I want to take the plasma to measure the miRNA in it. However, I found that multiple patients take blood transfusions before collecting the sample just in two days or a week so is it okay to keep their samples and work on it?

As far as I know, it is not advisable to use blood samples for miRNA analysis that have been collected shortly after a blood transfusion. Transfusion performed within 24 hours of the blood draw can significantly affect miRNA levels in plasma. For more than 48 hours before the blood draw, the effects on miRNA levels may be less pronounced Lugien Al-khalaileh.

Md. Niaz Morshed Ok, thank you very much