Is M-CSFR 1 a specific markers to identify the total population of macrophages?

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Malcolm Nobre

Hello Rosa Gallo

The major site of CSF1R expression is in macrophages. In hematopoiesis, CSF1R is upregulated during monocyte differentiation, but is downregulated during granulopoiesis. CSF1R is expressed predominantly in committed macrophage precursors, monocytes and tissue macrophages.

There are several macrophage cell surface markers which are commonly used, but the exact marker that you may have to use will depend on the condition of their local environment as macrophages exhibit phenotypic heterogeneity, and they are also able to modify their immunological response according to individual stimuli.

Best.

Rosa Gallo

Thank you Malcolm Nobre! Basically, I am looking at 'all' adipose tissue macrophages. I have tried to stain for F4/80 but this antibody doesn't work very well, so I have stained for CMSFR-1 which looks quite good.