Is it suitable to perform protein blast blast for consensus sequence generated by bioeditor by using forward and reverse strand of DNA after proper al

01 January 1970 1 9K Report

Is it suitable to perform protein blast blast for consensus sequence generated by bioeditor by using forward and reverse strand of DNA after proper alignment..

Susanta Roy

The consensus sequence generated by a bioeditor is typically derived from aligning and merging multiple DNA sequences. Based on the input sequences, this consensus sequence represents the most likely nucleotide at each position. I would suggest doing nucleotide BLAST (blastn) first and then going for protein BLAST (blastp) depending on the outcomes, if appropriate. Nucleotide BLAST compares nucleotide sequences against a nucleotide database and can provide information about similar DNA sequences.

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