Good day house.
Please I want to screen about 100 DNA samples extracted from bats' blood for Plasmodium and its relatives. For the PCR I need positive control. Is it right for me to extract DNA from a Malaria Parasite positive human blood sample as the positive control?
Thanks
No. The agents pathogenic for animals are genetically different from the plasmodia infecting humans.
Christian G Meyer Thanks very much. How do I go about the DNA to use as positive control for my PCR then since I am not using microscopy for any of the samples.
Please first read this article and others on the diversitty of plasmodia in bats:
Extensive diversity of malaria parasites circulating in Central African bats and monkeys.
Boundenga L, Ngoubangoye B, Mombo IM, Tsoubmou TA, Renaud F, Rougeron V, Prugnolle F.
Ecol Evol. 2018 Oct 5;8(21):10578-10586.
Mr Olayinka Sunday Okoh, you can not use DNA of human malaria parasite as control... Because the species of malaria parasite in bat is different from those in human.
No, the species will be different, although some may be very close. A review of the literature may provide what you need, contact the authors and local zoology people. Good Luck with your research.
- Badges
- Science method