What can go wrong extracting DNA from Blood spot on FTA paper using QIAamp DNA kit?

Good day house. I have issues extracting high quality DNA from Blood spot on FTA paper for PCR and Sanger Sequencing. 1. Unclotted blood samples were spotted on FTA paper, and gDNA was...

05 June 2019 4,286 8 View

What can made DNA/PCR product disappear after Cleaning?

I extracted gDNA from blood samples preserved on FTA paper using QIAamp DNA mini Kit and then ran PCR. The Agarose Gel Electrophoresis reveal expected band. To clean my DNA before Sequencing, I...

03 April 2019 4,053 3 View

Is it right to use DNA from Malaria Positive human blood sample a PCR positive control for Plasmodium from Wild e.g bat?

Good day house. Please I want to screen about 100 DNA samples extracted from bats' blood for Plasmodium and its relatives. For the PCR I need positive control. Is it right for me to extract DNA...

03 April 2019 7,585 5 View

What determines the concentration of MgCl2 in PCR?

I need to run PCR using various published primers. For one of the primers I intend to use BJ1 & BN2. For this primer a paper reported using 2.5ul of 10x PCR buffer containing 15mM MgCl2...

02 March 2019 8,926 8 View

What is the function of the Universal primer, Inosine, in an oligonucleotide primer?

Good day house. Please what is the function of the universal primer, Inosine, in a primer? I need to use the primer for PCR. If I don't include the Inosine in the primer as published what is the...

02 March 2019 364 6 View

How do I cite and or categorize a manual?

Good day house. I used R for some statistical analysis. I will like to know how to specifically cite it because from the citation given by 'R Core Team' the publication is a manual, not a book,...

01 February 2019 3,555 3 View

Impact Factor of Asian Journal of Advances in Agricultural Research?

Please does anyone know the Impact Factor of Asian Journal of Advances in Agricultural Research publish by Science Domain International. I have searched for it over and over but could not locate it.

10 November 2018 5,641 3 View

Can Filter Paper be used to collect blood for the extraction of DNA meant for downstream applications (PCR and Sanger Sequencing)?

Please, if FTA Classic Paper is unavailable, can the normal filter paper be used to collect blood samples in the field for storage and transportation to the laboratory where high quality DNA will...

10 November 2018 4,826 3 View

Is Science Domain genuine?

Good day house. Please is Science Domain a genuine or fake publisher? Are Asian Journal of Advances in Agricultural Research and Journal of Experimental Agriculture International genuine or...

09 October 2018 7,423 7 View

What is IUCN policy on sample size and lethal collection of Near Threatened Species?

I am to work with straw-coloured fruit bats, which is listed as A Near Threatened Specie by IUCN. I am aware of IUCN policy on species listed as "Threatened Species" but unaware of any policy on...

03 April 2018 2,200 0 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View