Olayinka Sunday Okoh

11 Questions 10 Answers 0 Followers

Questions related from Olayinka Sunday Okoh

What can go wrong extracting DNA from Blood spot on FTA paper using QIAamp DNA kit?

Good day house. I have issues extracting high quality DNA from Blood spot on FTA paper for PCR and Sanger Sequencing. 1. Unclotted blood samples were spotted on FTA paper, and gDNA was...

06 June 2019 4,346 8 View

Is it right to use DNA from Malaria Positive human blood sample a PCR positive control for Plasmodium from Wild e.g bat?

Good day house. Please I want to screen about 100 DNA samples extracted from bats' blood for Plasmodium and its relatives. For the PCR I need positive control. Is it right for me to extract DNA...

04 April 2019 7,624 5 View

What can made DNA/PCR product disappear after Cleaning?

I extracted gDNA from blood samples preserved on FTA paper using QIAamp DNA mini Kit and then ran PCR. The Agarose Gel Electrophoresis reveal expected band. To clean my DNA before Sequencing, I...

04 April 2019 4,107 3 View

What is the function of the Universal primer, Inosine, in an oligonucleotide primer?

Good day house. Please what is the function of the universal primer, Inosine, in a primer? I need to use the primer for PCR. If I don't include the Inosine in the primer as published what is the...

03 March 2019 407 6 View

What determines the concentration of MgCl2 in PCR?

I need to run PCR using various published primers. For one of the primers I intend to use BJ1 & BN2. For this primer a paper reported using 2.5ul of 10x PCR buffer containing 15mM MgCl2...

03 March 2019 8,999 8 View

How do I cite and or categorize a manual?

Good day house. I used R for some statistical analysis. I will like to know how to specifically cite it because from the citation given by 'R Core Team' the publication is a manual, not a book,...

02 February 2019 3,599 3 View

Impact Factor of Asian Journal of Advances in Agricultural Research?

Please does anyone know the Impact Factor of Asian Journal of Advances in Agricultural Research publish by Science Domain International. I have searched for it over and over but could not locate it.

11 November 2018 5,692 3 View

Can Filter Paper be used to collect blood for the extraction of DNA meant for downstream applications (PCR and Sanger Sequencing)?

Please, if FTA Classic Paper is unavailable, can the normal filter paper be used to collect blood samples in the field for storage and transportation to the laboratory where high quality DNA will...

11 November 2018 4,921 3 View

Is Science Domain genuine?

Good day house. Please is Science Domain a genuine or fake publisher? Are Asian Journal of Advances in Agricultural Research and Journal of Experimental Agriculture International genuine or...

10 October 2018 7,466 7 View

What is IUCN policy on sample size and lethal collection involving Near Threatened Species?

Please is there any IUCN policy on sample size and lethal collection involving Near Threatened Species? I am only aware of that of Threatened species, not Near Threatened which I have searched...

04 April 2018 1,240 0 View

What is IUCN policy on sample size and lethal collection of Near Threatened Species?

I am to work with straw-coloured fruit bats, which is listed as A Near Threatened Specie by IUCN. I am aware of IUCN policy on species listed as "Threatened Species" but unaware of any policy on...

04 April 2018 2,238 0 View