What can go wrong extracting DNA from Blood spot on FTA paper using QIAamp DNA kit?

Good day house. I have issues extracting high quality DNA from Blood spot on FTA paper for PCR and Sanger Sequencing. 1. Unclotted blood samples were spotted on FTA paper, and gDNA was...

05 June 2019 4,286 8 View

What can made DNA/PCR product disappear after Cleaning?

I extracted gDNA from blood samples preserved on FTA paper using QIAamp DNA mini Kit and then ran PCR. The Agarose Gel Electrophoresis reveal expected band. To clean my DNA before Sequencing, I...

03 April 2019 4,053 3 View

Is it right to use DNA from Malaria Positive human blood sample a PCR positive control for Plasmodium from Wild e.g bat?

Good day house. Please I want to screen about 100 DNA samples extracted from bats' blood for Plasmodium and its relatives. For the PCR I need positive control. Is it right for me to extract DNA...

03 April 2019 7,585 5 View

What determines the concentration of MgCl2 in PCR?

I need to run PCR using various published primers. For one of the primers I intend to use BJ1 & BN2. For this primer a paper reported using 2.5ul of 10x PCR buffer containing 15mM MgCl2...

02 March 2019 8,926 8 View

What is the function of the Universal primer, Inosine, in an oligonucleotide primer?

Good day house. Please what is the function of the universal primer, Inosine, in a primer? I need to use the primer for PCR. If I don't include the Inosine in the primer as published what is the...

02 March 2019 364 6 View

How do I cite and or categorize a manual?

Good day house. I used R for some statistical analysis. I will like to know how to specifically cite it because from the citation given by 'R Core Team' the publication is a manual, not a book,...

01 February 2019 3,555 3 View

Can Filter Paper be used to collect blood for the extraction of DNA meant for downstream applications (PCR and Sanger Sequencing)?

Please, if FTA Classic Paper is unavailable, can the normal filter paper be used to collect blood samples in the field for storage and transportation to the laboratory where high quality DNA will...

10 November 2018 4,826 3 View

Is Science Domain genuine?

Good day house. Please is Science Domain a genuine or fake publisher? Are Asian Journal of Advances in Agricultural Research and Journal of Experimental Agriculture International genuine or...

09 October 2018 7,423 7 View

What is IUCN policy on sample size and lethal collection involving Near Threatened Species?

Please is there any IUCN policy on sample size and lethal collection involving Near Threatened Species? I am only aware of that of Threatened species, not Near Threatened which I have searched...

03 April 2018 1,202 0 View

What is IUCN policy on sample size and lethal collection of Near Threatened Species?

I am to work with straw-coloured fruit bats, which is listed as A Near Threatened Specie by IUCN. I am aware of IUCN policy on species listed as "Threatened Species" but unaware of any policy on...

03 April 2018 2,200 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View