Impact Factor of Asian Journal of Advances in Agricultural Research?

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What can go wrong extracting DNA from Blood spot on FTA paper using QIAamp DNA kit?

Good day house. I have issues extracting high quality DNA from Blood spot on FTA paper for PCR and Sanger Sequencing. 1. Unclotted blood samples were spotted on FTA paper, and gDNA was...

05 June 2019 4,376 8 View

Is it right to use DNA from Malaria Positive human blood sample a PCR positive control for Plasmodium from Wild e.g bat?

Good day house. Please I want to screen about 100 DNA samples extracted from bats' blood for Plasmodium and its relatives. For the PCR I need positive control. Is it right for me to extract DNA...

03 April 2019 7,656 5 View

What can made DNA/PCR product disappear after Cleaning?

I extracted gDNA from blood samples preserved on FTA paper using QIAamp DNA mini Kit and then ran PCR. The Agarose Gel Electrophoresis reveal expected band. To clean my DNA before Sequencing, I...

03 April 2019 4,134 3 View

What determines the concentration of MgCl2 in PCR?

I need to run PCR using various published primers. For one of the primers I intend to use BJ1 & BN2. For this primer a paper reported using 2.5ul of 10x PCR buffer containing 15mM MgCl2...

02 March 2019 9,027 8 View

What is the function of the Universal primer, Inosine, in an oligonucleotide primer?

Good day house. Please what is the function of the universal primer, Inosine, in a primer? I need to use the primer for PCR. If I don't include the Inosine in the primer as published what is the...

02 March 2019 429 6 View

How do I cite and or categorize a manual?

Good day house. I used R for some statistical analysis. I will like to know how to specifically cite it because from the citation given by 'R Core Team' the publication is a manual, not a book,...

01 February 2019 3,629 3 View

Can Filter Paper be used to collect blood for the extraction of DNA meant for downstream applications (PCR and Sanger Sequencing)?

Please, if FTA Classic Paper is unavailable, can the normal filter paper be used to collect blood samples in the field for storage and transportation to the laboratory where high quality DNA will...

10 November 2018 4,955 3 View

Is Science Domain genuine?

Good day house. Please is Science Domain a genuine or fake publisher? Are Asian Journal of Advances in Agricultural Research and Journal of Experimental Agriculture International genuine or...

09 October 2018 7,493 7 View

What is IUCN policy on sample size and lethal collection involving Near Threatened Species?

Please is there any IUCN policy on sample size and lethal collection involving Near Threatened Species? I am only aware of that of Threatened species, not Near Threatened which I have searched...

03 April 2018 1,264 0 View

What is IUCN policy on sample size and lethal collection of Near Threatened Species?

I am to work with straw-coloured fruit bats, which is listed as A Near Threatened Specie by IUCN. I am aware of IUCN policy on species listed as "Threatened Species" but unaware of any policy on...

03 April 2018 2,267 0 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

How much area/acres of agriculture land can irrigate by 1 cusec of water?

Kindly response

08 August 2024 1,214 2 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Why is the Protocol for Preventing Postpartum Hemorrhage (https://pubmed.ncbi.nlm.nih.gov/36113283/ ) not being widely adopted?

If the placenta is delivered in squatting position within 3 minutes of the birth of the baby, postpartum hemorrhage is completely eliminated. It works every time. It is quite likely that if a...

05 August 2024 8,895 0 View