Can Filter Paper be used to collect blood for the extraction of DNA meant for downstream applications (PCR and Sanger Sequencing)?

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What can go wrong extracting DNA from Blood spot on FTA paper using QIAamp DNA kit?

Good day house. I have issues extracting high quality DNA from Blood spot on FTA paper for PCR and Sanger Sequencing. 1. Unclotted blood samples were spotted on FTA paper, and gDNA was...

05 June 2019 4,286 8 View

What can made DNA/PCR product disappear after Cleaning?

I extracted gDNA from blood samples preserved on FTA paper using QIAamp DNA mini Kit and then ran PCR. The Agarose Gel Electrophoresis reveal expected band. To clean my DNA before Sequencing, I...

03 April 2019 4,053 3 View

Is it right to use DNA from Malaria Positive human blood sample a PCR positive control for Plasmodium from Wild e.g bat?

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03 April 2019 7,585 5 View

What determines the concentration of MgCl2 in PCR?

I need to run PCR using various published primers. For one of the primers I intend to use BJ1 & BN2. For this primer a paper reported using 2.5ul of 10x PCR buffer containing 15mM MgCl2...

02 March 2019 8,926 8 View

What is the function of the Universal primer, Inosine, in an oligonucleotide primer?

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02 March 2019 364 6 View

How do I cite and or categorize a manual?

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01 February 2019 3,555 3 View

Impact Factor of Asian Journal of Advances in Agricultural Research?

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03 April 2018 1,202 0 View

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03 April 2018 2,200 0 View

What are the values needed for gabor filter's parameter for cifar10 data?

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Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

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How to calculate Total Flavonoid?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Samples to study circular dichroism (chirality)?

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How to accurately quantify chitin?

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Can anyone help with Schistosoma identification?

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RNA-seq DEGs downstream analysis and reporting in literature?

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What are the ranges of four diffterent poles of butterworth filter for ECG signal?

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Which polarizers are usually used to measure the birefringence of zebrafish muscles?

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Magne Neby

Hi! I'm also curious about this. Have you managed to find an answer to your question yet? E.g. if the normal filter paper is stored dry in silicagel in a way its not contaminated.

Meheretu Yonas

For years, we used pre-punched filter paper (LDA 22, Ploufragan, France) and it worked well. In one occasion we used Gert’s Japanese filter paper.

Meheretu Yonas Thank you.