hi .. I noticed that in some experimental conditions the mRNA levels of stat3-beta are alomst zero (after RT-PCR) while there is a good amount of protein from the same cell source.. can anyone help me with that??
Hi Haya,
Depending on the organism you work with, other regulatory factors may be at play. For example transcripts can be translated repeatedly, or after the amount of protein is satisfactory the transcript could be degraded to prevent over expression.
What organism do you work with? Do you know anything about the regulation of that particular gene/protein?
hey anna
i am working with mcf7 cell line (breast cancer)
it is known that stat3 beta is generated by alternative splicing and that stat3 alpha ( the other isoform) is the dominant protein in the cell. also, stat3 alpha needs stat3 beta for its phosphorylation and activation
the condition that i asked about was observed after stat3-alpha over expression .. is it possible that the over-expressed stat3 alpha made the translation of stat3-beta more effecient and as a result no mRNA was observed?
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