I don't have a cDNA kit right now. Further, if there is any protocol to synthesize cDNA from mRNA then which equipments will use. I have Dry bath and refrigerated ultracentrifuge.
Yes, but you need to have all the required chemicals along with your template.
1. M-MuLV Reverse Transcriptase (NEB: M0253S)
2. oligo(dT) or random hexamer mixture or specific primers
3. dNTPs
you can maintain the required incubation at 42 degrees for a 30mins to an hour and enzyme inactivation at 70 degrees for 5-10min using the dry bath.
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