Waqas Farooq

4 Questions 3 Answers 0 Followers

Questions related from Waqas Farooq

Can anyone send me the detailed protocol of lentiviral titer determination and its invitro transduction into Hela cell line or MSCs?

I have searched a lot of information from the published article but couldn't find valuable. As I have prepared the Lentiviral vector and stored it in -80 degree!

22 June 2024 3,356 1 View

How to transduce lentiviral vector into ThP1 macrophages and Mesenchymal stem cells?

Is there any authentic reference from where I can get appropriate information regarding this and anyone who has experienced any one of these cells' transduction?

28 May 2024 6,515 0 View

Is it possible to synthesize cDNA manually? if yes then what protocol should follow?

I don't have a cDNA kit right now. Further, if there is any protocol to synthesize cDNA from mRNA then which equipments will use. I have Dry bath and refrigerated ultracentrifuge.

05 November 2022 136 1 View

What is the best protocol of broth microdilution method to check antimicrobial activity of crude extracts of Plants?

I need protocol; 1. to dilute crude extract of plants 2. to perform an antimicrobial activity of extracts

04 March 2022 381 17 View