Until today, a good colored image is obtained using exclusively light. Electron microscope uses electrons and echograph uses ultrason. That gives only gray images.
Thanks Duco, for inviting me to follow this questions and perhaps to formulate a serious answer.
Dear Kebbab Zoubir,
OK, first of all I don't have any experience with an ecograph / echograph device (which IMHO and to my knowledge are ultrasound driven). If I remember correctly I have seen been transformed into color (artificially) e. g. in Doppler Ultrasound machines used in Medicine for DEMO of vessels in human body. It is a kind of (aequidensite) made by special software within the electronic parts of the machines (I see I haven't understood this quite well and therefore should dig into special sources). No "vital colors", though, but sufficient for diagnosis and demonstration of changes in blood flow etc....(if I remember correctly, usually red, blue, yellow).
Concerning EM (TEM, SEM): if we have to use electrons (since we would like to "resolve" structures; and Duco is right in saying...>only a shadow of the sample>) I guess that we'll end up anyway in "gray images" of the structures we are analysing. These images on the >>'screen' might be "green" (according to the display screen emitting green fluorescence from phosphorous [and other] particles deposited on the screen, interacting with/hit by the electrons [in TEM direct interaction with screen or negative film or nowadays digital camera target, in SEM electrons will be collected by a collector device, linked to a scintillator connected to a photomultiplier and recorded as scan lines on a monitor... naturally today an electronic digital image could be saved] as of my knowledge in "simple" words).
I am not aware of a (and don't think this could be solved within at least the next 10 years) which is capable of selecting .
The only way for now is to "color" black and white images from TEM and/or SEM - in former days with classical physical development in photography, nowadays also electronically (see. e.g. Stefan Diller's homepage(s) at
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
by means of "Falschfarben" = (cf. e.g. GOOGLE SEARCH: < equidensitometry and color > or also < equidensitometry and color and "electron microscopy".
This said, I apologize if my opinion and view of the matter might be incorrect or out of fashion... but I deplore that I was not able in my long lasting 'academic career' to establish in my EM-Lab.
Interested in looking forward to experts' opinions which hopefully will chime in soon, best wishes and regards, Wolfgang
Dhrubajyoti is right. Transmission electron microscopy gives an image that is merely a shadow of the sample. When imaging with back scattered electron or secondary electrons it is again a grayvalue image that is obtained.
Dear Dhrubajyoti Gupta and Duco Kramer,
You are absolutely right. The goal researched is how to resolve this problem similarly to the old films (black and white) which are colored later. Is it possible to find a suitable detector (for example) which can do this function for electron microscope or echographe. Of course, it is known that in the first apparatus, electron are used but in the second, ultrasound are rather utilized. The great problem is to modify or discover new detectors suitable for the signal used and capable to restore true color of the observed sample.
So you wonder if another electron frequency might lead to other images. Interesting idea, but i have no idea if that is possible. Maybe @wolfgang muss may know this?
Thanks Duco, for inviting me to follow this questions and perhaps to formulate a serious answer.
Dear Kebbab Zoubir,
OK, first of all I don't have any experience with an ecograph / echograph device (which IMHO and to my knowledge are ultrasound driven). If I remember correctly I have seen been transformed into color (artificially) e. g. in Doppler Ultrasound machines used in Medicine for DEMO of vessels in human body. It is a kind of (aequidensite) made by special software within the electronic parts of the machines (I see I haven't understood this quite well and therefore should dig into special sources). No "vital colors", though, but sufficient for diagnosis and demonstration of changes in blood flow etc....(if I remember correctly, usually red, blue, yellow).
Concerning EM (TEM, SEM): if we have to use electrons (since we would like to "resolve" structures; and Duco is right in saying...>only a shadow of the sample>) I guess that we'll end up anyway in "gray images" of the structures we are analysing. These images on the >>'screen' might be "green" (according to the display screen emitting green fluorescence from phosphorous [and other] particles deposited on the screen, interacting with/hit by the electrons [in TEM direct interaction with screen or negative film or nowadays digital camera target, in SEM electrons will be collected by a collector device, linked to a scintillator connected to a photomultiplier and recorded as scan lines on a monitor... naturally today an electronic digital image could be saved] as of my knowledge in "simple" words).
I am not aware of a (and don't think this could be solved within at least the next 10 years) which is capable of selecting .
The only way for now is to "color" black and white images from TEM and/or SEM - in former days with classical physical development in photography, nowadays also electronically (see. e.g. Stefan Diller's homepage(s) at
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
by means of "Falschfarben" = (cf. e.g. GOOGLE SEARCH: < equidensitometry and color > or also < equidensitometry and color and "electron microscopy".
This said, I apologize if my opinion and view of the matter might be incorrect or out of fashion... but I deplore that I was not able in my long lasting 'academic career' to establish in my EM-Lab.
Interested in looking forward to experts' opinions which hopefully will chime in soon, best wishes and regards, Wolfgang
Dear Wolfgang H. Muss;
Until our day, in my knowledge, all the apparatus of observation which utilize signals other than light give gray images. Of course, we can attribute an arbitrary color by programing. This is used for mapping a sample according to a given parameter (energy, temperature, defect, etc.)
Really, i acknowledge that there are difficulty of conception of new detectors which gives true colors. It is an interesting subject of research. In the same way as television which has debuted with black and white image, is it possible to reach this goal (colored image) in the future ?
Dont forget that the binary black/white signal of TV changed to triple binary signal that is perceived as color. It stays in fact a gray value emited in blue, red or green.
Dear Kebbab,
in my reply above (thank you all for your confidence in my explanations so far (:-)) ) I stated:
Dear Wolfgang;
If we see in the evolution of science, we can notice that human knowledge is in continual progress. Imagery domain is a formidable example. historically, this discipline are in continual progress. If we take the screen evolution, the humanity has debuted by a gray cathodique tube, the delta tube, then the inline technology. Recently, we assist to the apparition of the plasma and LED screen. The electronics take into acompte the defect of human receptors (eyes, oreille).
TV Images are then fabricated by illuminated points, lines and finally wefts. All these steps are converted in electrical signals. Audio signal is added and a video signal is obtained to be transmitted. In the reception, the video signal is treated, then the image is obtained on the screen. Therefore, we manipulate electrical signals to make image. TV color is made by three fundamental color (Red, Green, Blue "RGB") but converted in electrical signal by the camera. In the apparatus of observation (like SEM or ECHOGRAPH), signal image is obtained by the emission of electron or ultrasound from the point of impact of the incident beam similarly to the video signal. The obtained signal is detected using suitable detector, treated and directed to the screen. Therefore there are a great similitude between the two domains. As in the TV, is it possible to do the same ?
Dear Kebbab,
at least for this one ("possible in TV?") I have to leave profound and serious replies to experts in this field. I have understood your imagination, opinion and thoughts for sure, but it is out of my knowledge and experience to comment further on that matter. Sorry for this & thank you for your acknowledged contribution and thoughts....
Have a beautiful and recreative weekend, best regards, Wolfgang
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